Vuylsteke D R, Hughes J d'A, Rajab K
International Institute of Tropical Agriculture, PMB 5320, Ibadan, Nigeria.
Plant Protection Division, P.O. Box 1062, Zanzibar.
Plant Dis. 1998 Dec;82(12):1403. doi: 10.1094/PDIS.1998.82.12.1403B.
Symptoms resembling those of viral leaf streak disease, caused by banana streak badnavirus (BSV), were observed in May 1998 on two banana (Musa spp.) landraces grown from farmer-collected propagules in a farmer's field at Kiboje Uchukuni, Zanzibar. Those showing symptoms were "French plantain" cv. Mzuzu and "Cavendish" banana cv. Mtwike. Leaf symptoms were expressed as chlorotic streaks and blotches. Leaf samples were indexed by immunosorbent electron microscopy with BSV and cucumber mosaic cucumovirus (CMV) antibodies, using partially purified preparations (2). The two landraces tested positive for BSV, corroborating the occurrence of BSV in Zanzibar. In addition, cv. Mtwike was found to be coinfected with CMV. No other viruslike particles were seen by electron microscopy. Although BSV has been reported in Zanzibar (1), it was only from symptoms in the Musa field genebank at Kizimbani Research Station. BSV has been found in many Musa collections worldwide, particularly in the widely grown cv. Mysore. This report confirms the presence of BSV in farmers' fields and is also the first report of CMV infecting banana in Zanzibar. References: (1) A. J. Dabek and J. M. Waller. Trop. Pest Management 36:157, 1990. (2) M. Diekmann and C. A. J. Putter. Musa spp. FAO/IPGRI Technical Guidelines for the Safe Movement of Germplasm No. 15. FAO/IPGRI, Rome, Italy, 1996.
1998年5月，在桑给巴尔基博杰乌丘库尼一位农民田地中，由农民收集的繁殖体培育出的两个香蕉（芭蕉属）地方品种上，观察到了类似香蕉线条病毒（BSV）引起的病毒性叶条斑病的症状。出现症状的品种为“法国大蕉”品种姆祖祖和“卡文迪什”香蕉品种姆特维克。叶片症状表现为褪绿条纹和斑点。使用部分纯化制剂，通过免疫吸附电子显微镜，用BSV和黄瓜花叶黄瓜病毒（CMV）抗体对叶片样本进行检测（2）。这两个地方品种对BSV检测呈阳性，证实了桑给巴尔存在BSV。此外，发现品种姆特维克同时感染了CMV。电子显微镜未观察到其他类病毒颗粒。虽然桑给巴尔曾有过关于BSV的报道（1），但仅来自基津巴尼研究站的芭蕉属田间基因库中的症状。BSV在全球许多芭蕉属种质收集中都有发现，尤其是在广泛种植的品种迈索尔中。本报告证实了农民田地中存在BSV，也是桑给巴尔CMV感染香蕉的首次报道。参考文献：（1）A. J. 达贝克和J. M. 沃勒。《热带病虫害管理》36:157，1990年。（2）M. 迪克曼和C. A. J. 普特。芭蕉属。粮农组织/国际植物遗传资源研究所种质安全流动技术准则第15号。粮农组织/国际植物遗传资源研究所，意大利罗马，1996年。
