Shafer W M, Onunka V, Hitchcock P J
J Infect Dis. 1986 May;153(5):910-7. doi: 10.1093/infdis/153.5.910.
We examined the resistance of Neisseria gonorrhoeae to proteins prepared from the granules of human polymorphonuclear neutrophils (PMNs). We found that nearly isogenic strains differing in lipopolysaccharide subunit molecular weight also differed in levels of resistance to crude granule extracts. N. gonorrhoeae strain WS1 was at least 10-fold less resistant than the parental strain FA 102 to granule extracts. Surprisingly, strain WS1 did not differ from FA 102 in resistance to two isolated antimicrobial proteins obtained previously from extracts of human PMN granules. We used strain WS1 in assays that detected antimicrobial proteins in granule extracts, and we obtained at least two proteins with apparent molecular masses of 24-25.5 kilodaltons that exerted potent in vitro antigonococcal activity. We found that the ED50 (concentration of protein required to kill 50% of gonococci) against the strain WS1 was approximately 0.006 microgram of protein/ml, whereas the ED50 against the parental strain (FA 102) was approximately 0.4 microgram of protein/ml. Accordingly, alterations in lipopolysaccharide structure apparently caused a 66-fold decrease in gonococcal resistance to granule proteins. Our data suggest that gonococcal resistance to oxygen-independent antimicrobial systems of human PMNs may, in part, depend on the availability of certain lipopolysaccharide domains involved in recognition of the antimicrobial granule proteins described in this report.
我们检测了淋病奈瑟菌对从人多形核中性粒细胞（PMN）颗粒制备的蛋白质的抗性。我们发现，脂多糖亚基分子量不同的近等基因菌株对粗颗粒提取物的抗性水平也不同。淋病奈瑟菌WS1菌株对颗粒提取物的抗性至少比亲本菌株FA 102低10倍。令人惊讶的是，WS1菌株与FA 102在对先前从人PMN颗粒提取物中获得的两种分离的抗菌蛋白的抗性方面没有差异。我们使用WS1菌株进行检测颗粒提取物中抗菌蛋白的试验，并获得了至少两种表观分子量为24 - 25.5千道尔顿的蛋白，它们具有强大的体外抗淋球菌活性。我们发现，针对WS1菌株的ED50（杀死50%淋球菌所需的蛋白质浓度）约为0.006微克蛋白质/毫升，而针对亲本菌株（FA 102）的ED50约为0.4微克蛋白质/毫升。因此，脂多糖结构的改变显然导致淋球菌对颗粒蛋白的抗性降低了66倍。我们的数据表明，淋球菌对人PMN非氧依赖性抗菌系统的抗性可能部分取决于某些参与识别本报告中所述抗菌颗粒蛋白的脂多糖结构域的可用性。
