Casey S G, Shafer W M, Spitznagel J K
Infect Immun. 1985 Feb;47(2):401-7. doi: 10.1128/iai.47.2.401-407.1985.
We investigated the in vitro resistance of Neisseria gonorrhoeae FA19 to the O2-independent antimicrobial systems of human polymorphonuclear leukocytes. Acid extracts of polymorphonuclear leukocyte granules (crude granule extracts) and a purified granule protein (57 kilodaltons) were, at low concentrations, bactericidal for gonococci under aerobic conditions that permitted growth. However, they were less effective under anaerobic conditions that imposed bacteriostasis. We found that adding sodium nitrite to reduced growth media permitted the growth of strain FA19 in an anaerobic environment. Under these conditions with nitrite, anaerobic cultures of strain FA19 were no more resistant to the crude granule extract and the 57-kilodalton protein than aerobic cultures. In contrast, Salmonella typhimurium SL-1004, a facultative anaerobe, was readily killed by both the crude granule extract and the 57-kilodalton antimicrobial protein regardless of the presence or absence of free molecular oxygen. This is the first demonstration that an isolated antimicrobial protein from polymorphonuclear leukocyte granules is active against bacteria under anaerobic conditions. Our results also indicated that the efficacy of human polymorphonuclear leukocyte O2-independent killing of N. gonorrhoeae may, in part, be inhibited by bacteriostatic conditions imposed by hypoxia.
我们研究了淋病奈瑟菌FA19对人多形核白细胞非氧依赖抗菌系统的体外耐药性。多形核白细胞颗粒的酸性提取物（粗颗粒提取物）和一种纯化的颗粒蛋白（57千道尔顿）在低浓度下，在允许淋病奈瑟菌生长的需氧条件下对其具有杀菌作用。然而，在导致抑菌作用的厌氧条件下，它们的效果较差。我们发现向减少营养成分的培养基中添加亚硝酸钠可使FA19菌株在厌氧环境中生长。在有亚硝酸盐的这些条件下，FA19菌株的厌氧培养物对粗颗粒提取物和57千道尔顿蛋白的耐药性并不比需氧培养物更强。相比之下，兼性厌氧菌鼠伤寒沙门氏菌SL - 1004，无论有无游离分子氧，都很容易被粗颗粒提取物和57千道尔顿抗菌蛋白杀死。这是首次证明从多形核白细胞颗粒中分离出的一种抗菌蛋白在厌氧条件下对细菌具有活性。我们的结果还表明，人多形核白细胞对淋病奈瑟菌的非氧依赖杀伤作用的效力可能部分受到缺氧导致的抑菌条件的抑制。
