Doenecke D, Tönjes R
J Mol Biol. 1986 Feb 5;187(3):461-4. doi: 10.1016/0022-2836(86)90446-8.
A human H1 histone gene and its flanking sequences were isolated from a human gene library using a fragment of the duck H5 histone gene as a hybridization probe. The primary structure of this human H1 histone (as deduced from the nucleotide sequence of the gene) reveals a close homology of H1 and H5 histones and fits the three-domain organization of all members of the H1 histone family. Within this protein organization, the C-terminal domain of H1 differs from the arginine-rich H5 in its distribution of the basic amino acids: the C-terminal domain of the human H1 shows only one arginine and most of the H5 specific arginine positions show lysine instead.
利用鸭H5组蛋白基因片段作为杂交探针，从人基因文库中分离出一个人H1组蛋白基因及其侧翼序列。该人H1组蛋白的一级结构（由基因的核苷酸序列推导得出）显示H1和H5组蛋白具有高度同源性，并且符合H1组蛋白家族所有成员的三结构域组织形式。在这种蛋白质结构中，H1的C末端结构域在碱性氨基酸分布上与富含精氨酸的H5不同：人H1的C末端结构域仅显示一个精氨酸，而H5特有的大多数精氨酸位置则为赖氨酸。
