Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana.
Wiley Interdiscip Rev RNA. 2019 Jul;10(4):e1529. doi: 10.1002/wrna.1529. Epub 2019 Mar 7.
Processing and maturation of precursor RNA species is coupled to RNA polymerase II transcription. Co-transcriptional RNA processing helps to ensure efficient and proper capping, splicing, and 3' end processing of different RNA species to help ensure quality control of the transcriptome. Many improperly processed transcripts are not exported from the nucleus, are restricted to the site of transcription, and are in some cases degraded, which helps to limit any possibility of aberrant RNA causing harm to cellular health. These critical quality control pathways are regulated by the highly dynamic protein-protein interaction network at the site of transcription. Recent work has further revealed the extent to which the processes of transcription and RNA processing and quality control are integrated, and how critically their coupling relies upon the dynamic protein interactions that take place co-transcriptionally. This review focuses specifically on the intricate balance between 3' end processing and RNA decay during transcription termination. This article is categorized under: RNA Turnover and Surveillance > Turnover/Surveillance Mechanisms RNA Processing > 3' End Processing RNA Processing > Splicing Mechanisms RNA Processing > Capping and 5' End Modifications.
前体 RNA 种类的加工和成熟与 RNA 聚合酶 II 转录相偶联。共转录 RNA 加工有助于确保不同 RNA 种类的有效和适当的加帽、剪接和 3' 端加工，以帮助确保转录组的质量控制。许多加工不当的转录本不会从核内输出，被限制在转录部位，在某些情况下会被降解，这有助于限制任何异常 RNA 对细胞健康造成伤害的可能性。这些关键的质量控制途径受转录部位高度动态的蛋白质-蛋白质相互作用网络调控。最近的工作进一步揭示了转录和 RNA 加工和质量控制过程整合的程度，以及它们的偶联在多大程度上依赖于共转录时发生的动态蛋白质相互作用。这篇综述特别关注在转录终止过程中 3' 端加工和 RNA 降解之间的复杂平衡。本文属于以下分类：RNA 周转和监控 > 周转/监控机制 RNA 加工 > 3' 端加工 RNA 加工 > 剪接机制 RNA 加工 > 加帽和 5' 端修饰。
