Nordmann Birthe, Schilling Tobias, Hoppert Michael, Hertel Robert
Department of Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August-University Göttingen, Göttingen, Germany.
Department of General Microbiology, Institute of Microbiology and Genetics, Georg-August-University Göttingen, Göttingen, Germany.
Arch Virol. 2019 May;164(5):1485-1488. doi: 10.1007/s00705-019-04187-z. Epub 2019 Mar 8.
Bacillus thuringiensis (Bt) is non-pathogenic for humans and serves as a biological control agent in agriculture. Understanding its phages will help to prevent industrial production loss of Bt products and will lead to a better understanding of phages in general. The complete genome of the new B. thuringiensis phage isolate vB_BthM-Goe5 (Goe5) was sequenced, revealing a linear 157,804-bp-long dsDNA chromosome flanked by 2579-bp-long terminal repeats. It contains two tRNAs and 272 protein coding regions, 69 of which could be assigned with an annotation. Morphological investigation, using transmission electron microscopy, revealed Myoviridae morphology. The formation of a double baseplate upon tail sheath contraction indicates a link to the group of SPO1-related phages. Comparative genomics with all Bacillus-related viral genomes available in the NCBI genome database during this investigation indicated that Goe5 was a unique isolate, with Bacillus phage Bastille as its closest relative.
苏云金芽孢杆菌（Bt）对人类无致病性，在农业中用作生物防治剂。了解其噬菌体将有助于防止Bt产品的工业生产损失，并有助于更全面地了解噬菌体。对新的苏云金芽孢杆菌噬菌体分离株vB_BthM-Goe5（Goe5）的全基因组进行了测序，结果显示其线性双链DNA染色体长度为157,804 bp，两侧为长度为2579 bp的末端重复序列。它包含两个tRNA和272个蛋白质编码区，其中69个可以进行注释。使用透射电子显微镜进行的形态学研究揭示了该噬菌体属于肌尾噬菌体科形态。尾鞘收缩时形成双基板表明它与SPO1相关噬菌体组有关。在本次研究中，与NCBI基因组数据库中所有可用的芽孢杆菌相关病毒基因组进行比较基因组学分析表明，Goe5是一个独特的分离株，与芽孢杆菌噬菌体巴士底狱最为接近。
