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噬菌体 vB_BveM-Goe7 代表了巴氏噬菌体亚科中的一个新属。

Phage vB_BveM-Goe7 represents a new genus in the subfamily Bastillevirinae.

机构信息

Department of Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August-University Göttingen, Göttingen, Germany.

Department of General Microbiology, Institute of Microbiology and Genetics, Georg-August-University Göttingen, Göttingen, Germany.

出版信息

Arch Virol. 2020 Apr;165(4):959-962. doi: 10.1007/s00705-020-04546-1. Epub 2020 Feb 12.

Abstract

Bacillus velezensis FZB42 is a Gram-positive, endospore-forming rhizobacterium that is associated with plant roots and promotes plant growth. It was used as host to isolate phage vB_BveM-Goe7 (Goe7). Goe7 exhibits a Myoviridae morphology with a contractile tail and an icosahedral head. Its genome is 158,674 bp in size and contains 5137-bp-long terminal repeats (LTRs). It also contains five tRNA-encoding genes and 251 coding DNA sequences (CDS), of which 65 were annotated. The adsorption constant of Goe7 is 6.1 ± 0.24 × 10 ml/min, with a latency period of 75 min and a burst size of 114 particles per burst. A BLASTn sequence comparison against the non-redundant nucleotide database of NCBI revealed that Goe7 is most similar to Bacillus subtilis phage vB_BsuM-Goe3.

摘要

贝莱斯芽孢杆菌 FZB42 是一种革兰氏阳性、形成芽孢的根际细菌,与植物根系相关,能促进植物生长。它被用作宿主来分离噬菌体 vB_BveM-Goe7(Goe7)。Goe7 呈现出肌尾噬菌体的形态,具有收缩尾和二十面体头部。其基因组大小为 158674bp,包含 5137bp 长的末端重复序列(LTRs)。它还包含五个 tRNA 编码基因和 251 个编码 DNA 序列(CDS),其中 65 个被注释。Goe7 的吸附常数为 6.1±0.24×10ml/min,潜伏期为 75min,爆发量为每个爆发 114 个颗粒。与 NCBI 的非冗余核苷酸数据库进行 BLASTn 序列比较表明,Goe7 与枯草芽孢杆菌噬菌体 vB_BsuM-Goe3 最为相似。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd30/7131981/a52b582f8b5d/705_2020_4546_Fig1_HTML.jpg

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