Laboratory of Bacteriophage Biology, Skryabin Institute of Biochemistry and Physiology of Microorganisms, RAS, 142290 Pushchino, Russia.
Department of Microbiology, Federal State Budgetary Educational Institution of Higher Education "Vyatka State University", 610000 Kirov, Russia.
Viruses. 2019 Jul 7;11(7):624. doi: 10.3390/v11070624.
The group of bacteria includes, inter alia, the species known to be associated with human diseases and food poisoning. Here, we describe the phage vB_BtS_B83 (abbreviated as B83) infecting the species of this group. Transmission electron microscopy (TEM) micrographs indicate that B83 belongs to the family. B83 is a temperate phage using an arbitrium system for the regulation of the lysis-lysogeny switch, and is probably capable of forming a circular plasmid prophage. Comparative analysis shows that it has been previously sequenced, but was mistaken for a plasmid. B83 shares common genome organization and >46% of proteins with other the phage, BMBtp14. Phylograms constructed using large terminase subunits and a pan-genome presence-absence matrix show that these phages form a clade distinct from the closest viruses. Based on the above, we propose the creation of a new genus named that includes B83 and BMBtp14.
该细菌群包括已知与人类疾病和食物中毒有关的物种。在这里，我们描述了一种感染该群组物种的噬菌体 vB_BtS_B83（简称 B83）。透射电子显微镜（TEM）显微照片表明，B83 属于 科。B83 是一种温和噬菌体，使用仲裁系统来调节裂解-溶原开关，并且可能能够形成环状质粒原噬菌体。比较分析表明，它以前已经被测序，但被误认为是质粒。B83 与其他噬菌体，BMBtp14 共享共同的基因组组织和 >46%的蛋白质。使用大型终止酶亚基和泛基因组存在缺失矩阵构建的系统发育树表明，这些噬菌体形成了一个与最接近的病毒不同的分支。基于以上结果，我们建议创建一个新的属，命名为 ，其中包括 B83 和 BMBtp14。
