通过离子淌度-质谱联用技术（IM-MS）对小分子药物及类药物分子进行异构体和构象分析。
Isomeric and Conformational Analysis of Small Drug and Drug-Like Molecules by Ion Mobility-Mass Spectrometry (IM-MS).
作者信息
Phillips Shawn T, Dodds James N, May Jody C, McLean John A
机构信息
Department of Chemistry, Center for Innovative Technology, Vanderbilt Institute of Chemical Biology, Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt-Ingram Cancer Center, Vanderbilt University, Nashville, TN, USA.
出版信息
Methods Mol Biol. 2019;1939:161-178. doi: 10.1007/978-1-4939-9089-4_9.
Abstract
This chapter provides a broad overview of ion mobility-mass spectrometry (IM-MS) and its applications in separation science, with a focus on pharmaceutical applications. A general overview of fundamental ion mobility (IM) theory is provided with descriptions of several contemporary instrument platforms which are available commercially (i.e., drift tube and traveling wave IM). Recent applications of IM-MS toward the evaluation of structural isomers are highlighted and placed in the context of both a separation and characterization perspective. We conclude this chapter with a guided reference protocol for obtaining routine IM-MS spectra on a commercially available uniform-field IM-MS.
摘要
本章对离子淌度-质谱联用技术（IM-MS）及其在分离科学中的应用进行了广泛概述，重点介绍了其在药物领域的应用。文中提供了基本离子淌度（IM）理论的概述，并描述了几种市售的当代仪器平台（即漂移管离子淌度和行波离子淌度）。突出了IM-MS在评估结构异构体方面的最新应用，并从分离和表征的角度进行了阐述。本章最后给出了一个指导性参考方案，用于在市售的均匀场IM-MS上获取常规的IM-MS谱图。
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