Ghobadi Hassan, Alipour Mohammad Reza, Keyhanmanesh Rana, Boskabady Mohammad Hossein, Aslani Mohammad Reza
Department of Internal Medicine, Pulmonary Division, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Iran J Allergy Asthma Immunol. 2019 Feb;18(1):48-61.
Epidemiological and clinical studies have demonstrated a close association between obesity and asthma. The current study investigated the effect of high-fat diet on tracheal responsiveness to methacholine and insulin resistance in ovalbumin (OVA) sensitized male and female rats. The rats were divided into eight groups (n=6 per group): female with the normal diet (F+ND), male with the normal diet (M+ND), female OVA-sensitized with the normal diet (F+SND), male OVA-sensitized with the normal diet (M+SND), female with high-fat diet (F+HFD), male with high-fat diet (M+HFD), female OVA-sensitized with high-fat diet (F+SHFD), and male OVA-sensitized with high-fat diet (M+SHFD). All rats were fed for 8 weeks with high-fat diet or standard pelts, and for another 4 weeks, they were sensitized with OVA or saline. At the end of the study, the tracheal responsiveness to methacholine, serum insulin, and blood glucose levels was measured. Also, insulin resistance indexes were determined. OVA-sensitization and diet-induced obesity caused the curve of methacholine concentration response to shifting to the left. In addition, results indicated that the EC50 (the effective concentration of methacholine generating 50% of peak response) in F+SHFD rats was statistically lower than M+SHFD group (p<0.05). Moreover, insulin resistance was higher in the F+SHFD than the M+SHFD group (p<0.001). These results suggest that insulin resistance and metabolic syndrome may be involved in the pathogenesis of obesity associated with OVA-sensitized rats condition, especially in female animals.
流行病学和临床研究表明，肥胖与哮喘之间存在密切关联。本研究调查了高脂饮食对卵清蛋白（OVA）致敏的雄性和雌性大鼠气管对乙酰甲胆碱的反应性及胰岛素抵抗的影响。将大鼠分为八组（每组n = 6）：正常饮食雌性组（F + ND）、正常饮食雄性组（M + ND）、正常饮食OVA致敏雌性组（F + SND）、正常饮食OVA致敏雄性组（M + SND）、高脂饮食雌性组（F + HFD）、高脂饮食雄性组（M + HFD）、高脂饮食OVA致敏雌性组（F + SHFD）和高脂饮食OVA致敏雄性组（M + SHFD）。所有大鼠用高脂饮食或标准饲料喂养8周，然后再用OVA或生理盐水致敏4周。在研究结束时，测量气管对乙酰甲胆碱的反应性、血清胰岛素和血糖水平。此外，还测定了胰岛素抵抗指数。OVA致敏和饮食诱导的肥胖导致乙酰甲胆碱浓度反应曲线向左移动。此外，结果表明，F + SHFD组大鼠的EC50（产生50%峰值反应的乙酰甲胆碱有效浓度）在统计学上低于M + SHFD组（p < 0.05）。而且，F + SHFD组的胰岛素抵抗高于M + SHFD组（p < 0.001）。这些结果表明，胰岛素抵抗和代谢综合征可能参与了与OVA致敏大鼠状况相关的肥胖发病机制，尤其是在雌性动物中。
