Hesampour Fateme, Namavar Jahromi Bahia, Tahmasebi Foroozan, Gharesi-Fard Behrouz
Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran.
Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran AND Department of Gynecology and Obstetrics, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran J Allergy Asthma Immunol. 2019 Feb;18(1):91-99.
Polycystic ovary syndrome (PCOS) is correlated with low-grade chronic inflammation. Interleukin-17A (IL-17A) and Interleukin-32 (IL-32) are two members of the pro-inflammatory cytokines which act as significant components of the immune system during certain inflammatory diseases. Along with immunological processes, genetic factors play major roles in predisposition to PCOS. There are myriad single nucleotide polymorphisms (SNPs) within IL-17A and IL-32 genes that may affect their production and the susceptibility of individuals to PCOS. The objective of the present research was to investigate the association between IL-17A (rs2275913) and IL-32 (rs9927163, rs4786370) SNPs, and also their serum levels with susceptibility to PCOS in a group of Iranian women. In this case-control study, 150 PCOS patients (mean age of 29.1 years) and 150 healthy women (mean age of 26.1 years) were analyzed in terms of IL-17A and IL-32 SNPs via polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Furthermore, serum levels of IL-17A and IL-32 cytokines were measured through the use of ELISA method. There were significant differences between PCOS and healthy women regarding IL-17A rs2275913 alleles, genotypes frequencies (p=0.005, and 0.01, respectively) and the allelic distribution of IL-32 rs9927163 SNP (p=0.03). Additionally, significant differences were indicated between two groups concerning the AG genotype against AA+GG genotypes (p=0.009) and the GG genotype against AA+AG genotypes (p=0.006) in IL-17A rs2275913 SNP. In the matter of IL-32 gene SNPs, GC haplotype frequency was significantly different between patients and controls (p=0.05). Furthermore, IL-32 serum level was not significantly different between the two studied groups and the serum level of IL-17A was not detectable. In conclusion, IL-17A and IL-32 SNPs might be associated with predisposition to PCOS in Iranian women.
多囊卵巢综合征（PCOS）与低度慢性炎症相关。白细胞介素-17A（IL-17A）和白细胞介素-32（IL-32）是促炎细胞因子中的两个成员，在某些炎症性疾病中作为免疫系统的重要组成部分发挥作用。除免疫过程外，遗传因素在PCOS易感性中起主要作用。IL-17A和IL-32基因内存在无数单核苷酸多态性（SNP），可能影响它们的产生以及个体对PCOS的易感性。本研究的目的是调查一组伊朗女性中IL-17A（rs2275913）和IL-32（rs9927163、rs4786370）SNP及其血清水平与PCOS易感性之间的关联。在这项病例对照研究中，通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术对150例PCOS患者（平均年龄29.1岁）和150名健康女性（平均年龄26.1岁）的IL-17A和IL-32 SNP进行了分析。此外，通过酶联免疫吸附测定（ELISA）方法测量了IL-17A和IL-32细胞因子的血清水平。PCOS患者和健康女性在IL-17A rs2275913等位基因、基因型频率（分别为p = 0.005和0.01）以及IL-32 rs9927163 SNP的等位基因分布方面存在显著差异（p = 0.03）。此外，在IL-17A rs2275913 SNP中，AG基因型与AA + GG基因型（p = 0.009）以及GG基因型与AA + AG基因型（p = 0.006）之间的两组差异也很显著。关于IL-32基因SNP，患者和对照组之间的GC单倍型频率存在显著差异（p = 0.05）。此外，两个研究组之间的IL-32血清水平没有显著差异，且未检测到IL-17A的血清水平。总之，IL-17A和IL-32 SNP可能与伊朗女性的PCOS易感性有关。
