Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Microb Pathog. 2020 Jul;144:104195. doi: 10.1016/j.micpath.2020.104195. Epub 2020 Apr 12.
Hepatitis B virus (HBV) approximately infects 350 million people. Interleukin-17 (IL-17) as a pro-inflammatory cytokine, have been found to modulate the immune system in infectious and inflammatory diseases. Recently, the influence of genetic changes like single nucleotide polymorphisms (SNP) on expression rate and function of cytokine has been widely investigated. This study was performed to determine any possible association between four IL-17 SNPs (rs2397084, rs763780, rs2275913 and rs10484879) and chronic HBV infection. A total of 466 samples were recruited and studied including 199 chronic patients, 172 healthy controls and 95 spontaneous clearance individuals between genotype and allele frequencies. Genomic DNA was extracted from peripheral blood cells and Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP) was used to determine the SNPs genotypes. Genotypes frequencies for rs10484879 were 63.8% CC, 31.7% AC, 4.5% AA in chronic group, 54.7% CC, 36.6% AC, 8.7% AA in control and 63.2% CC, 33.7% AC, 5.8% AA in cleared samples. The AC genotype for rs10484879 was significantly associated with a decreased risk of HBV chronicity (Pvalue = 0.031, OR = 2.699, 95%CI: 1.097-6.639). The genotype and allele frequencies of rs2397084, rs763780 and rs2275913 did not show significant difference between chronic HBV patients and healthy controls. Indeed, there is no significant difference between clearance and chronic patient's genotypes in four SNPs. Our results suggest that IL-17A rs10484879 single nucleotide polymorphism genotype is probably associated with susceptibility to HBV chronic infection, while no significant differences in IL-17 rs2397084, rs763780 and rs227591 distribution were found between HBV patients and spontaneous clearance individuals and control participants.
乙型肝炎病毒（HBV）感染约 3.5 亿人。白细胞介素-17（IL-17）作为一种促炎细胞因子，已被发现可调节感染和炎症性疾病中的免疫系统。最近，人们广泛研究了单核苷酸多态性（SNP）等遗传变化对细胞因子表达率和功能的影响。本研究旨在确定 IL-17 四个 SNP（rs2397084、rs763780、rs2275913 和 rs10484879）与慢性 HBV 感染之间是否存在任何关联。共招募了 466 例样本，包括 199 例慢性患者、172 例健康对照者和 95 例自发清除者，分析基因型和等位基因频率。从外周血单个核细胞中提取基因组 DNA，采用聚合酶链反应限制性片段长度多态性（PCR-RFLP）方法检测 SNP 基因型。慢性组 rs10484879 基因型频率为 63.8%CC、31.7%AC、4.5%AA，对照组为 54.7%CC、36.6%AC、8.7%AA，清除组为 63.2%CC、33.7%AC、5.8%AA。rs10484879 的 AC 基因型与 HBV 慢性化的风险降低显著相关（P 值=0.031，OR=2.699，95%CI：1.097-6.639）。rs2397084、rs763780 和 rs2275913 的基因型和等位基因频率在慢性 HBV 患者和健康对照者之间无显著差异。实际上，在这四个 SNP 中，清除者和慢性患者的基因型之间没有显著差异。我们的研究结果表明，白细胞介素-17A rs10484879 单核苷酸多态性基因型可能与 HBV 慢性感染的易感性相关，而 HBV 患者与自发清除者和对照组参与者之间，白细胞介素-17 rs2397084、rs763780 和 rs227591 的分布无显著差异。
