Baharlou Rasoul, Rashidi Nesa, Ahmadi-Vasmehjani Abbas, Khoubyari Mahshid, Sheikh Maryam, Erfanian Saiedeh
Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran AND Department of Immunology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Iran J Allergy Asthma Immunol. 2019 Feb;18(1):114-119.
Adipose-derived mesenchymal stem cells (Ad-MSCs) have been reported to suppress the effector T cell responses and have beneficial effects on various immune disorders, like rheumatoid arthritis (RA). This study was designed to investigate the effects of co-cultured Ad-MSCs on peripheral blood mononuclear cells (PBMCs) of RA patients and healthy individuals, through assessing transcription factors of T cell subsets. PBMCs from RA patients and healthy donors were co-cultured with Ad-MSCs with or without Phytohaemagglutinin (PHA). The quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the expression of T-box 21 (T-bet), GATA-binding protein-3 (GATA3), retinoid-related orphan receptor γt (ROR-γt) and forkhead box P3 (Foxp3). Based on the results, Ad-MSCs greatly upregulated Th2 and Treg cell transcription factors, i.e., GATA3 and Foxp3 (p<0.05), and downregulated Th1 and Th17 transcription factors, i.e., T-bet and RORγt (p<0.05). These results demonstrate that Ad-MSCs can result in an immunosuppressive environment through inhibition of pro-inflammatory T cells and induction of T cells with a regulatory phenotype. Therefore, they might have important clinical implications for inflammatory and autoimmune diseases such as RA.
据报道，脂肪来源的间充质干细胞（Ad-MSCs）可抑制效应T细胞反应，并对多种免疫疾病，如类风湿性关节炎（RA）具有有益作用。本研究旨在通过评估T细胞亚群的转录因子，研究共培养的Ad-MSCs对RA患者和健康个体外周血单个核细胞（PBMCs）的影响。将RA患者和健康供体的PBMCs与添加或不添加植物血凝素（PHA）的Ad-MSCs共培养。采用定量实时聚合酶链反应（qRT-PCR）检测T-box 21（T-bet）、GATA结合蛋白3（GATA3）、维甲酸相关孤儿受体γt（ROR-γt）和叉头框P3（Foxp3）的表达。结果显示，Ad-MSCs显著上调Th2和Treg细胞转录因子，即GATA3和Foxp3（p<0.05），并下调Th1和Th17转录因子，即T-bet和RORγt（p<0.05）。这些结果表明，Ad-MSCs可通过抑制促炎性T细胞和诱导具有调节表型的T细胞，营造免疫抑制环境。因此，它们可能对RA等炎症性和自身免疫性疾病具有重要的临床意义。
