支持细胞中FSH依赖性蛋白对睾丸间质细胞活性的旁分泌调控：一项体外研究
Paracrine control of Leydig cell activity by FSH dependent proteins from Sertoli cells: an in vitro study.
作者信息
Benahmed M, Tabone E, Grenot C, Sanchez P, Chauvin M A, Morera A M
出版信息
J Steroid Biochem. 1986 Jan;24(1):311-5. doi: 10.1016/0022-4731(86)90071-3.
The regulating effect of follicle-stimulating hormone (FSH) on Leydig cell function was studied using a model of immature porcine Leydig and Sertoli cells cultured in a hormone supplemented defined medium. FSH pretreatment for 2 days of Leydig cells cultured alone was with no effect. FSH pretreatment of Leydig cells cocultured with Sertoli cells increases Leydig cell activity in an FSH dose-dependent manner with a maximal effect observed at 50 ng/ml porcine FSH (pFSH). Leydig cells cultured for 2 days in conditioned medium (CM) by FSH stimulated (FSH-CM) Sertoli cells, as compared to CM by unstimulated (control) (C-CM) Sertoli cells show an increase of their activity with a maximal effect observed at 50 ng/ml pFSH. Leydig cells cultured in CM as compared to non CM, show a marked development of organelles (smooth endoplasmic reticulum and mitochondria) involved in the steroidogenic activity. The activity of FSH-CM as compared to C-CM on Leydig cell function was non dialyzable and trypsin sensitive. These data suggest that Sertoli cells exert a regulatory action on Leydig cell steroidogenic activity via FSH dependent secreted proteins.
使用在添加激素的限定培养基中培养的未成熟猪睾丸间质细胞和支持细胞模型，研究了促卵泡激素（FSH）对睾丸间质细胞功能的调节作用。单独培养的睾丸间质细胞经FSH预处理2天没有效果。与支持细胞共培养的睾丸间质细胞经FSH预处理后，睾丸间质细胞活性以FSH剂量依赖的方式增加，在50 ng/ml猪促卵泡激素（pFSH）时观察到最大效应。与未受刺激（对照）（C-CM）支持细胞的条件培养基（CM）相比，经FSH刺激（FSH-CM）的支持细胞培养2天的条件培养基培养的睾丸间质细胞，其活性增加，在50 ng/ml pFSH时观察到最大效应。与非CM相比，在CM中培养的睾丸间质细胞显示出参与类固醇生成活性的细胞器（滑面内质网和线粒体）有明显发育。与C-CM相比，FSH-CM对睾丸间质细胞功能的活性不可透析且对胰蛋白酶敏感。这些数据表明，支持细胞通过FSH依赖性分泌蛋白对睾丸间质细胞类固醇生成活性发挥调节作用。
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