卵泡刺激素通过支持细胞蛋白调节培养的睾丸间质细胞功能：一项体外研究。

FSH regulates cultured Leydig cell function via Sertoli cell proteins: an in vitro study.

作者信息

Benahmed M, Grenot C, Tabone E, Sanchez P, Morera A M

出版信息

Biochem Biophys Res Commun. 1985 Oct 30;132(2):729-34. doi: 10.1016/0006-291x(85)91193-3.

Abstract

The effects of follicular stimulating hormone (FSH) on testicular steroidogenic activity has been studied by testing the capacity of conditioned medium (CM) by both unstimulated (control) Sertoli cells (C-CM) and FSH stimulated Sertoli cells (FSH-CM) to influence porcine cultured Leydig cell activity. Leydig cells cultured in FSH-CM for 48 hrs, as compared to C-CM, show a significant (P less than 0.05) increase in [125I]-hCG binding (150% +/- 4) and hCG-stimulated testosterone (T) secretion (266% +/- 42). In addition, the stimulating effect of FSH-CM on Leydig cell function as compared to C-CM, is trypsin sensitive, non dialyzable, heat stable, acid resistant and is chromatographed following gel filtration (Sephadex G 100) into two different peaks of activity. These data suggest that FSH regulates Leydig cell function via (at least two types of) Sertoli cell secreted proteins.

摘要

通过检测未受刺激（对照）的支持细胞（C-CM）和促卵泡激素（FSH）刺激的支持细胞（FSH-CM）的条件培养基（CM）影响猪培养的睾丸间质细胞活性的能力，研究了FSH对睾丸类固醇生成活性的影响。与C-CM相比，在FSH-CM中培养48小时的睾丸间质细胞，其[125I]-人绒毛膜促性腺激素（hCG）结合能力显著增加（P<0.05）（150%±4），hCG刺激的睾酮（T）分泌增加（266%±42）。此外，与C-CM相比，FSH-CM对睾丸间质细胞功能的刺激作用对胰蛋白酶敏感、不可透析、热稳定、耐酸，经凝胶过滤（葡聚糖G 100）层析后分为两个不同的活性峰。这些数据表明，FSH通过（至少两种类型的）支持细胞分泌的蛋白质调节睾丸间质细胞功能。