Center for Natural Product Technologies (CENAPT), Program for Collaborative Research in the Pharmaceutical Sciences (PCRPS), and Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy , University of Illinois at Chicago , Chicago , Illinois 60612 , United States.
Department of Physical Sciences, Rosary College of Arts and Sciences , Dominican University , River Forest , Illinois 60305 , United States.
J Nat Prod. 2019 Mar 22;82(3):621-630. doi: 10.1021/acs.jnatprod.9b00020. Epub 2019 Mar 8.
Much uncertainty exists in science and herbal products referencing turmeric (T), turmeric extract (TE), curcuminoid-enriched turmeric extract (CTE), further processed curcuminoid-enriched materials (CEM), or curcumin as a single-chemical entity. To facilitate the rational chemical and biological assessment of turmeric-derived NPs, we introduced the DESIGNER approach of Depleting and Enriching Select Ingredients to Generate Normalized Extract Resources to Curcuma longa preparations. Countercurrent separation of a commercial CTE yielded four key materials-lipophilic metabolites; purified curcumin ("purcumin"); a mixture of curcumin, demethoxycurcumin, and bisdemethoxycurcumin ("purcuminoids"); and hydrophilic metabolites-and enabled production of a curcuminoid-free TE ("nocumin"). Their characterization utilized TLC, H (q)NMR spectroscopy, and HPLC.
科学和草药产品中存在许多不确定性，涉及姜黄（T）、姜黄提取物（TE）、富含姜黄素的姜黄提取物（CTE）、进一步加工的富含姜黄素的材料（CEM）或姜黄素作为单一化学实体。为了促进对姜黄衍生纳米颗粒进行合理的化学和生物学评估，我们引入了一种方法，即通过去除和富集选择成分来生成标准化提取物资源，以用于姜黄制剂。对商业 CTE 的逆流分离得到了四种关键材料 - 亲脂性代谢物；纯化姜黄素（“purcumin”）；姜黄素、脱甲氧基姜黄素和双脱甲氧基姜黄素的混合物（“姜黄素类”）；以及亲水性代谢物 - 并能够生产不含姜黄素的 TE（“nocumin”）。它们的特性利用 TLC、H（q）NMR 光谱和 HPLC 进行了表征。
