Programa de Pós-Graduação em Biotecnologia e Biociências, Universidade Federal de Santa Catarina, Florianópolis CEP:88040-970, Brazil.
Departamento de Química, Universidade Federal de Santa Catarina, Florianópolis, Brazil.
Ecotoxicol Environ Saf. 2019 Jun 15;174:334-343. doi: 10.1016/j.ecoenv.2019.01.130. Epub 2019 Mar 5.
Ulva ohnoi is a green macroalga with fast growth and high rates of nitrogen and phosphorus absorption. Recently, this species has been recorded in several places with record green tide formation in some of them. Using molecular tools, we herein report the first occurrence of this species in Brazil and demonstrate its potential for phytoremediation in typical environmental concentrations of Cd (0.625-15 µg L). Similarly, the effects of physicochemical parameters (salinity and temperature) on the toxicity and uptake efficiency of this species were evaluated. Molecular analysis of two sequences (1141 bp) obtained corroborates another 34 sequences for U. ohnoi obtained from GenBank. The addition of Cd in the medium affected photosynthetic parameters and reduced growth rate. U. ohnoi showed resistance to Cd when cultivated at 18 °C, S15 and 18-25 °C, S35, at concentrations between 0.625 and 2.5 μg. L of Cd; yet, positive growth rate was maintained. Dose-dependent accumulation was observed in all combinations of factors used with a maximum value of 4.20 μg Cd per gram of dry seaweed at 15 μg. L of Cd at 18 °C and S35. Maximum value of the concentration factor was 81.3 ± 1.1% of Cd added at the concentration of 0.625 μg. L to S15 and 18 °C. Our results demonstrate the potential of using U. ohnoi in the phytoremediation of Cd in saltwater or brackish water.
大青藤是一种生长迅速、氮磷吸收效率高的绿藻。最近，该物种已在多个地方被记录到，其中一些地方出现了绿潮。本文利用分子工具首次报道了该物种在巴西的发生情况，并证明了其在典型环境浓度 Cd（0.625-15μg/L）下进行植物修复的潜力。同样，本文还评估了理化参数（盐度和温度）对该物种毒性和吸收效率的影响。对获得的两个序列（1141bp）进行分子分析，与从 GenBank 获得的另外 34 个 U. ohnoi 序列相吻合。在培养基中添加 Cd 会影响光合作用参数并降低生长速度。当在 18°C、S15 和 18-25°C、S35 下培养时，U. ohnoi 对 Cd 表现出抗性，浓度在 0.625 到 2.5μg/L 之间；然而，仍保持正生长速率。在所有使用的因素组合中观察到剂量依赖性积累，在 15μg/L Cd 和 18°C、S35 下，最大 Cd 积累值为每克干海藻 4.20μg。在 0.625μg/L Cd 和 S15、18°C 时，Cd 加入浓度的浓度因子最大值为 81.3±1.1%。我们的结果表明，U. ohnoi 在海水或微咸水中 Cd 的植物修复中具有潜力。
