Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Biosens Bioelectron. 2019 Apr 15;131:250-256. doi: 10.1016/j.bios.2019.02.007. Epub 2019 Feb 21.
In this work, by using amorphous bimetallic sulfides of CoSnSPd as signal amplifier and magnetic material of FeO @PPyAu as the substrate, we fabricated an electrochemical immunoassay for Nterminal prohormone of brain natriuretic peptide (NTpro BNP). CoSnS was used as the signal amplifier of the immunosensor for the first time, which was synthesized by fast stoichiometric co-precipitation method. The binary sulfide was identified as an amorphous structure and performed apparent electrochemical behavior. The excellent performance of signal marker was induced by the synergistic effect between SnS and cobalt, as well as the loaded Pd nanoparticles (NPs). Both CoSnS and Pd NPs presented excellent electrocatalytic activity toward HO oxidation. Moreover, the magnetic nanocomposite of FeO @PPyAu was firmly immobilized on the magnetic glassy carbon electrode (MGCE) by the magnetic force. The magnetic substrate not only performed good electron conductivity, but also improved the stability of the fabricated electrochemical immunosensor. The developed immunosensor for the NTpro BNP detection exhibited a wide linear response (0.1 pg/mL to 50 ng/mL), and low detection limit of 31.5 fg/mL. In addition, the immunosensor held an excellent analysis capability and broad fascinating application for the detection of other biomarkers in medical diagnosis and treatment.
在这项工作中，我们使用非晶态双金属硫化物 CoSnSPd 作为信号放大器和 FeO@PPyAu 作为基底的磁性材料，制备了一种用于脑钠肽前体 (NTproBNP) 的电化学免疫分析。首次将 CoSnS 用作免疫传感器的信号放大器，通过快速化学计量共沉淀法合成。二元硫化物被鉴定为非晶态结构，并表现出明显的电化学行为。信号标记的优异性能是由 SnS 和钴的协同作用以及负载的 Pd 纳米颗粒 (NPs) 引起的。CoSnS 和 Pd NPs 对 HO 氧化均表现出优异的电催化活性。此外，FeO@PPyAu 的磁性纳米复合材料通过磁力牢固地固定在磁性玻碳电极 (MGCE) 上。磁性基底不仅具有良好的导电性，而且提高了所制备的电化学免疫传感器的稳定性。用于检测 NTproBNP 的开发的免疫传感器表现出宽的线性响应范围（0.1 pg/mL 至 50 ng/mL），检测限低至 31.5 fg/mL。此外，该免疫传感器具有出色的分析能力，为医学诊断和治疗中其他生物标志物的检测提供了广阔的应用前景。
