Li Yueyuan, Liu Lei, Liu Xuejing, Ren Yong, Xu Kun, Zhang Nuo, Sun Xiaojun, Yang Xinglong, Ren Xiang, Wei Qin
Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China.
Department of Mathematical Sciences, Zibo Normal College, Zibo, Shandong, 255130, China.
Biosens Bioelectron. 2020 Sep 1;163:112280. doi: 10.1016/j.bios.2020.112280. Epub 2020 May 12.
A method for the detection of procalcitonin (PCT) with improved accuracy is urgently needed. A dual-mode electrochemical immunosensor with CuCoS-Au bimetallic sulfides as enhancer for accurate detection of PCT was fabricated in this work. The immunosensor not only displays a heavy square wave voltammetry (SWV) signal due to the electron transfer of Cu and Cu but not the addition of electron mediators. But also presents high electrocatalytic activity towards HO redox reactions and enhances the detection sensitivity of the amperometric i-t curve (i-t). The introduction of Au nanoparticles, not only improves the conductivity but also has synergistic effects between Au NPs and CuCoS, which promote the detection sensitivity by SWV and i-t. To firmly immobilize antibodies, electrolytic gold was used as the matrix for the immunosensor. Based on a CuCoS-Au dual-signal indicator and electrolytic gold as the matrix, the developed immunosensor for PCT detection in this study demonstrates a wide linear response (0.0001-50 ng/mL) and a low detection limit (SWV: 82.6 fg/mL and i-t: 95.4 fg/mL). Additionally, the fabricated immunosensor exhibited an outstanding analytical approach for PCT and broad application for other biomarkers in clinical diagnosis and treatment.
迫切需要一种提高降钙素原（PCT）检测准确性的方法。在这项工作中，制备了一种以CuCoS-Au双金属硫化物为增强剂的双模式电化学免疫传感器，用于准确检测PCT。该免疫传感器不仅由于Cu和Cu的电子转移而显示出强烈的方波伏安法（SWV）信号，而且无需添加电子媒介体。此外，它对HO氧化还原反应具有高电催化活性，并提高了安培i-t曲线（i-t）的检测灵敏度。金纳米颗粒的引入不仅提高了导电性，而且Au NPs与CuCoS之间具有协同作用，通过SWV和i-t提高了检测灵敏度。为了牢固固定抗体，将电解金用作免疫传感器的基质。基于CuCoS-Au双信号指示剂和以电解金为基质，本研究开发的用于PCT检测的免疫传感器显示出宽线性响应（0.0001-50 ng/mL）和低检测限（SWV：82.6 fg/mL和i-t：95.4 fg/mL）。此外，所制备的免疫传感器对PCT表现出出色的分析方法，并在临床诊断和治疗中对其他生物标志物具有广泛的应用。
