Weldon School of Biomedical Engineering, Birck Nanotechnology Center, Center for Implantable Device, Purdue University, West Lafayette, IN, USA.
School of Mechanical Engineering, Purdue University, West Lafayette, IN, USA.
Biosens Bioelectron. 2019 Apr 15;131:257-266. doi: 10.1016/j.bios.2019.01.051. Epub 2019 Jan 31.
Glutamate excitotoxicity is a pathology in which excessive glutamate can cause neuronal damage and degeneration. It has also been linked to secondary injury mechanisms in traumatic spinal cord injury. Conventional bioanalytical techniques used to characterize glutamate levels in vivo, such as microdialysis, have low spatiotemporal resolution, which has impeded our understanding of this dynamic event. In this study, we present an amperometric biosensor fabricated using a simple direct ink writing technique for the purpose of in vivo glutamate monitoring. The biosensor is fabricated by immobilizing glutamate oxidase on nanocomposite electrodes made of platinum nanoparticles, multi-walled carbon nanotubes, and a conductive polymer on a flexible substrate. The sensor is designed to measure extracellular dynamics of glutamate and other potential biomarkers during a traumatic spinal cord injury event. Here we demonstrate good sensitivity and selectivity of these rapidly prototyped implantable biosensors that can be inserted into a spinal cord and measure extracellular glutamate concentration. We show that our biosensors exhibit good flexibility, linear range, repeatability, and stability that are suitable for future in vivo evaluation.
谷氨酸兴奋性毒性是一种病理学，其中过量的谷氨酸会导致神经元损伤和变性。它也与创伤性脊髓损伤的继发性损伤机制有关。传统的生物分析技术，如微透析，用于体内特征化谷氨酸水平，具有低时空分辨率，这阻碍了我们对这种动态事件的理解。在这项研究中，我们提出了一种使用简单的直接墨水书写技术制造的电流型生物传感器，用于体内谷氨酸监测。该生物传感器通过将谷氨酸氧化酶固定在由铂纳米粒子、多壁碳纳米管和导电聚合物组成的纳米复合材料电极上，在柔性基底上制造而成。该传感器旨在测量创伤性脊髓损伤事件期间的细胞外谷氨酸和其他潜在生物标志物的动力学。在这里，我们展示了这些快速原型化的植入式生物传感器具有良好的灵敏度和选择性，可以插入脊髓并测量细胞外谷氨酸浓度。我们表明，我们的生物传感器具有良好的柔韧性、线性范围、重复性和稳定性，适合未来的体内评估。
