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四种实验室培养酵母和两种注册生物防治产品对梨采后腐烂的控制

Control of Postharvest Decay in Pear by Four Laboratory-Grown Yeasts and Two Registered Biocontrol Products.

作者信息

Sugar David, Spotts Robert A

机构信息

Oregon State University, Southern Oregon Research and Extension Center, 569 Hanley Rd., Medford 97502.

Oregon State University, Mid-Columbia Agricultural Research and Extension Center, Hood River 97031.

出版信息

Plant Dis. 1999 Feb;83(2):155-158. doi: 10.1094/PDIS.1999.83.2.155.

Abstract

Control of blue mold decay in Bosc pears was studied with the laboratory-grown yeasts Rhodotorula glutinis, Cryptococcus infirmo-miniatus, and two strains of Cryptococcus laurentii, as well as registered biocontrol products Aspire, containing the yeast Candida oleophila, and Bio-Save 11 (now Bio-Save 110), containing the bacterium Pseudomonas syringae. Both thiabendazole (TBZ)-sensitive and TBZ-resistant strains of Penicillium expansum were used. Aspire treatment reduced the average lesion diameter by approximately 65 and 45%, and reduced decay incidence by 27 and 9% with TBZ-resistant and TBZ-sensitive P. expansum, respectively, in the first year of the study, but did not result in significant decay control in the second year. Bio-Save 11 reduced decay lesion diameter by 32 to 72% and incidence by 21 to 40% over the 2 years. In both years, TBZ-sensitive P. expansum was completely controlled by the combination of either C. laurentii (both strains), R. glutinis, or C. infirmo-miniatus with 100 ppm TBZ. With TBZ-resistant P. expansum, control of wound infection with these yeasts alone or with 100 ppm TBZ ranged from 62.9 to 100%. In a packinghouse trial, control by Bio-Save 110 + 100 ppm TBZ and Aspire + 100 ppm TBZ was not different than control by TBZ at 569 ppm, the maximum label rate. The amount of decay following Aspire + 100 ppm TBZ treatment was significantly less than the amount of decay following Bio-Save 110 + 100 ppm TBZ treatment.

摘要

利用实验室培养的酵母——粘红酵母、浅小隐球酵母以及两种罗伦隐球酵母菌株,还有已注册的生物防治产品——含有嗜油假丝酵母的Aspire和含有丁香假单胞菌的Bio - Save 11(现Bio - Save 110),研究了它们对博斯克梨青霉腐烂病的防治效果。使用了对噻菌灵(TBZ)敏感和耐药的扩展青霉菌株。在研究的第一年,Aspire处理使TBZ耐药和TBZ敏感的扩展青霉的平均病斑直径分别减小了约65%和45%,腐烂发生率分别降低了27%和9%,但在第二年并未实现显著的腐烂控制效果。在两年时间里,Bio - Save 11使腐烂病斑直径减小了32%至72%,发病率降低了21%至40%。在这两年中,TBZ敏感的扩展青霉通过罗伦隐球酵母(两种菌株)、粘红酵母或浅小隐球酵母与100 ppm TBZ的组合实现了完全控制。对于TBZ耐药的扩展青霉,单独使用这些酵母或与100 ppm TBZ联合使用对伤口感染的控制率在62.9%至100%之间。在一个包装厂试验中,Bio - Save 110 + 100 ppm TBZ和Aspire + 100 ppm TBZ的防治效果与569 ppm(标签最大用量)的TBZ防治效果没有差异。Aspire + 100 ppm TBZ处理后的腐烂量显著低于Bio - Save 110 + 100 ppm TBZ处理后的腐烂量。

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