Holopainen I, Kontro P
Neurochem Res. 1986 Feb;11(2):207-15. doi: 10.1007/BF00967969.
The kinetics and specificity of taurine and beta-alanine uptake were studied in primary cultures of rat astrocytes under identical experimental conditions. The uptake consisted of nonsaturable penetration and saturable high-affinity transport that was strictly sodium dependent. The cells accumulated taurine more effectively than beta-alanine, both the affinity and uptake capacity being greater for taurine. Taurine uptake was competitively inhibited by beta-alanine and GABA, the former being more potent. Also, hypotaurine and 2-guanidinoethanesulphonic acid strongly reduced taurine uptake, but L-2,4-diaminobutyric acid had no significant effect. beta-Alanine uptake was also competitively inhibited by GABA, but the most potent inhibitors were hypotaurine and 2-guanidinoethanesulphonic acid. L-2,4-Diaminobutyric acid was moderately active. The uptake systems for taurine and beta-alanine were thus in principle similar, and they exhibited certain characteristics typical for a neurotransmitter amino acid. The inhibition studies further suggest the existence of only one common transport system for taurine, beta-alanine, and GABA in cultured primary astrocytes. The same uptake system may also be used for hypotaurine.
在相同实验条件下，对原代培养的大鼠星形胶质细胞中牛磺酸和β-丙氨酸摄取的动力学及特异性进行了研究。摄取包括非饱和渗透和严格依赖钠的饱和高亲和力转运。细胞积累牛磺酸比β-丙氨酸更有效，牛磺酸的亲和力和摄取能力都更高。牛磺酸摄取受到β-丙氨酸和GABA的竞争性抑制，前者作用更强。此外，亚牛磺酸和2-胍基乙磺酸强烈降低牛磺酸摄取，但L-2,4-二氨基丁酸无显著影响。β-丙氨酸摄取也受到GABA的竞争性抑制，但最有效的抑制剂是亚牛磺酸和2-胍基乙磺酸。L-2,4-二氨基丁酸活性中等。因此，牛磺酸和β-丙氨酸的摄取系统原则上相似，且表现出神经递质氨基酸的某些典型特征。抑制研究进一步表明，在培养的原代星形胶质细胞中，牛磺酸、β-丙氨酸和GABA仅存在一种共同转运系统。同一摄取系统也可用于亚牛磺酸。
