Holopainen I, Malminen O, Kontro P
Department of Biomedical Sciences, University of Tampere, Finland.
J Neurosci Res. 1987;18(3):479-83. doi: 10.1002/jnr.490180315.
Taurine uptake in cultured cerebellar granule cells and astrocytes consisted of a saturable high-affinity component and nonsaturable diffusion. The transport constant (Km) was significantly lower and the maximal velocity (V) higher in granule cells than in astrocytes. The uptakes were strictly sodium dependent and also moderately decreased in potassium-free medium. The specificity profile of taurine uptake was similar in both cell types, hypotaurine, beta-alanine, and guanidinoethanesulphonic acid being the most potent inhibitors, followed by GABA and homotaurine. Glutamate inhibited taurine uptake more in astrocytes than in granule cells. In principle, the uptake systems were similar in granule cells and astrocytes, exhibiting features characteristic of uptake of a neurotransmitter or -modulator.
培养的小脑颗粒细胞和星形胶质细胞对牛磺酸的摄取包括一个可饱和的高亲和力成分和非饱和扩散。颗粒细胞中的转运常数（Km）显著低于星形胶质细胞，而最大速度（V）则高于星形胶质细胞。摄取严格依赖于钠，并且在无钾培养基中也适度降低。两种细胞类型中牛磺酸摄取的特异性谱相似，次牛磺酸、β-丙氨酸和胍基乙磺酸是最有效的抑制剂，其次是GABA和高牛磺酸。谷氨酸对星形胶质细胞中牛磺酸摄取的抑制作用比对颗粒细胞更强。原则上，颗粒细胞和星形胶质细胞中的摄取系统相似，表现出神经递质或调节剂摄取的特征。
