Kontro P
Neuroscience. 1983 Jan;8(1):153-9. doi: 10.1016/0306-4522(83)90034-9.
beta-[3H]Alanine uptake by mouse brain slices was studied in Krebs-Ringer-HEPES-glucose medium (pH 7.4) under O2. The uptake was temperature-sensitive and consisted of two saturable transport components, high- and low-affinity, with kinetic parameters comparable to those of amino acid neurotransmitter candidates. beta-Alanine uptake was strictly sodium-dependent and also inhibited by the omission of potassium and presence of ouabain, suggesting that the transport is mainly fuelled by cation gradients. Sodium ions showed positive cooperative effects in beta-alanine uptake, indicating the association of at least two sodium ions in the transfer of one molecule of beta-alanine. The uptake was strongly inhibited by gamma-aminobutyrate and hypotaurine, the high-affinity uptake component completely disappearing in the presence of hypotaurine. Taurine had no measurable effect. The results suggest that the high-affinity transports of beta-alanine and hypotaurine may be mediated by the same system.
在氧气条件下，于Krebs-Ringer-HEPES-葡萄糖培养基（pH 7.4）中研究了小鼠脑片对β-[³H]丙氨酸的摄取。该摄取对温度敏感，由两个可饱和转运成分组成，即高亲和力和低亲和力成分，其动力学参数与氨基酸神经递质候选物的参数相当。β-丙氨酸摄取严格依赖于钠离子，并且在缺钾和存在哇巴因的情况下也受到抑制，这表明转运主要由阳离子梯度驱动。钠离子在β-丙氨酸摄取中显示出正协同效应，表明在一分子β-丙氨酸的转运中至少有两个钠离子参与。γ-氨基丁酸和亚牛磺酸强烈抑制摄取，在亚牛磺酸存在下高亲和力摄取成分完全消失。牛磺酸没有可测量的影响。结果表明，β-丙氨酸和亚牛磺酸的高亲和力转运可能由同一系统介导。
