Mahmood T, Rush C M
Texas Agricultural Experiment Station, P.O. Drawer 10, Bushland 79012.
Plant Dis. 1999 Jun;83(6):521-526. doi: 10.1094/PDIS.1999.83.6.521.
A high degree of reciprocal cross-protection occurred in sugar beets between beet soilborne mosaic virus (BSBMV) and beet necrotic yellow vein virus (BNYVV) in greenhouse experiments. The incidence of cross-protection depended on the interval between inoculations with protecting and challenging virus; longer inoculation intervals enhanced the incidence of cross-protection. Cross-protection was most effective when the inoculation interval was between 5 and 10 days, a period during which virus accumulated to a maximum level in plants singly infected with BSBMV or BNYVV. Results obtained by enzyme-linked immunosorbent assay (ELISA) and Western blotting were consistent and indicated that cross-protection affected viral capsid protein accumulation. RNA of both protecting and challenging viruses was detected in doubly infected plants by using reverse transcription-polymerase chain reaction (RT-PCR), indicating that RNA of the challenge virus was present in protected plants, but capsid protein was undetected by serological tests. BSBMV is a closely related but serologically distinct virus that provides an excellent system for studies of cross-protection with BNYVV.
在温室试验中，甜菜土传花叶病毒（BSBMV）和甜菜坏死黄脉病毒（BNYVV）之间在甜菜上发生了高度的相互交叉保护作用。交叉保护的发生率取决于接种保护病毒和攻击病毒之间的间隔时间；接种间隔时间越长，交叉保护的发生率越高。当接种间隔在5至10天之间时，交叉保护最为有效，在此期间，病毒在单独感染BSBMV或BNYVV的植物中积累到最高水平。通过酶联免疫吸附测定（ELISA）和蛋白质免疫印迹法获得的结果一致，表明交叉保护影响病毒衣壳蛋白的积累。通过逆转录-聚合酶链反应（RT-PCR）在双重感染的植物中检测到了保护病毒和攻击病毒的RNA，这表明攻击病毒的RNA存在于受保护的植物中，但血清学检测未检测到衣壳蛋白。BSBMV是一种密切相关但血清学上不同的病毒，它为研究与BNYVV的交叉保护提供了一个极好的系统。
