Wisler G C, Widner J N, Duffus J E, Liu H-Y, Sears J L
USDA-ARS, 1636 E. Alisal St. Salinas, CA 93905.
Southern Minnesota Beet Sugar Cooperative, E. Hwy 212, P.O. Box 500, Renville 56284.
Plant Dis. 1997 Feb;81(2):229. doi: 10.1094/PDIS.1997.81.2.229D.
Several fields planted in sugar beet (Beta vulgaris) in the Southern Minnesota Beet Sugar Cooperative growing area showed patches of pale greenish yellow foliage and upright leaves characteristic of rhizomania. Other symptoms included reduced root size and root proliferation. Samples taken from these areas during August of 1996 were evaluated for beet necrotic yellow vein virus (BNYVV), the cause of rhizomania, and for other sugar beet furoviruses. BNYVV was identified in 59 of 90 beet samples tested with enzyme-linked immunosorbent assay and Western blot (immunoblot) analyses (molecular mass approximately 22 kDa) with specific (polyclonal antisera donated by K. Richards; monoclonal antisera donated by G. Grassi) and broadly reactive antisera produced at the USDA in Salinas, CA. Recovery by mechanical inoculation of Chenopodium quinoa and Beta macrocarpa confirmed identity. Beet leaves showing symptoms of vein clearing, vein banding, mosaic, and vein necrosis were all identified as being infected with beet soilborne mosaic virus (BSBMV). No systemic leaf symptoms of BNYVV were found in any sample. The BSBMV isolates were identical to one another based on symptomatology of indicator plants and molecular masses in Western blots (approximately 24 kDa), but symptoms were distinct from those of other members of the BSBMV serogroup isolates previously studied from Texas, Idaho, Nebraska, and Colorado. The beet soilborne virus (BSBV) was also recovered by mechanical inoculation and Western blot analysis (antisera donated by R. Koenig) in three samples of field-collected beets. This is a new report of BNYVV, BSBMV, and BSBV in Minnesota. The distribution of rhizomania in infested fields was not isolated to any general area, which indicates that the virus has been present and multiplying in previous sugar beet crops and was not detected. Severity of infection ranged from mild root symptoms with near normal yields and sugar content, to moderate and severe root symptoms with low yields and low sugar content.
明尼苏达州南部甜菜糖业合作社种植区的几块种植甜菜（Beta vulgaris）的田地出现了一片片淡黄绿色叶子且叶片直立的症状，这是根肿病的典型特征。其他症状包括根部尺寸减小和根系增生。1996年8月从这些区域采集的样本被检测是否感染了导致根肿病的甜菜坏死黄脉病毒（BNYVV）以及其他甜菜真菌传病毒。使用酶联免疫吸附测定和蛋白质印迹（免疫印迹）分析（分子量约22 kDa），用特异性（由K. Richards捐赠的多克隆抗血清；由G. Grassi捐赠的单克隆抗血清）以及美国农业部在加利福尼亚州萨利纳斯生产的广泛反应性抗血清，对90个甜菜样本中的59个进行了检测，确定了BNYVV的存在。通过机械接种藜麦（Chenopodium quinoa）和大果甜菜（Beta macrocarpa）进行病毒回接，证实了病毒的身份。表现出叶脉黄化、叶脉带状、花叶和叶脉坏死症状的甜菜叶片均被鉴定为感染了甜菜土壤传播花叶病毒（BSBMV）。在任何样本中均未发现BNYVV的系统性叶片症状。基于指示植物的症状学和蛋白质印迹中的分子量（约24 kDa），BSBMV分离株彼此相同，但症状与先前从得克萨斯州、爱达荷州、内布拉斯加州和科罗拉多州研究的BSBMV血清群分离株的其他成员不同。通过机械接种和蛋白质印迹分析（由R. Koenig捐赠抗血清），在三个田间采集的甜菜样本中也检测到了甜菜土壤传播病毒（BSBV）。这是明尼苏达州关于BNYVV、BSBMV和BSBV的新报告。根肿病在受侵染田地中的分布并不局限于任何特定区域，这表明该病毒在之前的甜菜作物中已经存在并繁殖，但未被检测到。感染的严重程度从根部症状较轻、产量和含糖量接近正常，到根部症状中度和重度、产量和含糖量较低不等。
