Department of Chemistry, Gastroenterology and Hepatology, Zhongshan Hospital, Institutes of Biomedical Sciences and Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200433, China.
Department of Gastroenterology, Zhongshan Hospital of Fudan University, Shanghai 200032, China.
J Chromatogr A. 2019 Jun 21;1595:1-10. doi: 10.1016/j.chroma.2019.02.039. Epub 2019 Feb 18.
Both of phosphorylation and glycosylation are playing crucial roles in biological processes. For deep analysis of phosphorylation and glycosylation, a multifunctional nanocomposite (dubbed Mag TiO-GSH) is constructed by coating hydrophilic tripeptide on the surface of magnetic titania. The introduction of titanium oxide and hydrophilic tripeptide endows the nanocomposite specific affinity preference towards phospho- and glycopeptides. Besides, the employment of magnetic nanoparticles dramatically simplifies the solid-liquid separation process. Combining all above mentioned performance of Mag TiO-GSH nanocomposites with mass spectrometry technique, a multipurpose platform is built for the universal enrichment of phospho- and glycopeptides. In addition to the high sensitivity and selectivity, they also perform excellent in biological samples. Thus, a total of 975 phosphopeptides and 1243 glycopeptides are identified from merely 100 μg mouse brain extracts at the same time, which will make it possible to exceedingly advance the phospho- and glycoproteomics and simplify the screening of related biomarkers.
磷酸化和糖基化在生物过程中都起着至关重要的作用。为了深入分析磷酸化和糖基化，通过在磁性氧化钛表面涂覆亲水性三肽，构建了一种多功能纳米复合材料（称为 MagTiO-GSH）。氧化钛和亲水性三肽的引入赋予了纳米复合材料对磷酸肽和糖肽的特定亲和偏好。此外，磁性纳米粒子的使用极大地简化了固液分离过程。将 MagTiO-GSH 纳米复合材料的所有上述性能与质谱技术相结合，构建了一个通用的用于磷酸肽和糖肽富集的多功能平台。除了高灵敏度和选择性外，它们在生物样品中也表现出色。因此，仅从 100μg 小鼠脑组织提取物中同时鉴定出 975 个磷酸肽和 1243 个糖肽，这将使磷酸化和糖蛋白质组学的研究取得极大进展，并简化相关生物标志物的筛选。
