Department of Chemistry and The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, 200433, China.
Institutes of Biomedical Sciences and Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai, 200433, China.
Mikrochim Acta. 2019 Feb 4;186(3):159. doi: 10.1007/s00604-019-3274-3.
A hydrophilic material consisting of a magnetite core coated with mercaptosuccinic acid modified mesoporous titania (denoted as FeO@mTiO-MSA) has been fabricated. It is shown to be a viable sorbent for capturing glycopeptides and phosphopeptides. The sorbent combines the features of metal oxide-based affinity chromatography and of hydrophilic interaction liquid chromatography (HILIC) with the advantages of using mesoporous titania. The use of magnetic microspheres provides magnetic response and simplifies separation. Following elution with 10% ammonia, the peptides were submitted to LC-MS/MS analysis. The method enabled 327 phosphopeptides and 65 glycopeptides to be identified in three isolated replicates of merely 5 μL samples of human saliva. Among them, the phosphorylation sites and glycosylation sites were detected in 20 peptide segments. Graphical abstract Schematic presentation of preparation of novel hydrophilic magnetic mesoporous titania nanomaterials (FeO@mTiO-MSA). This specific sorbent exhibits highly selective and efficient simultaneous adsorption ability for both glycopeptides and phosphopeptides from biosamples by mass spectrometric analysis.
一种由磁性氧化铁核表面包覆巯基琥珀酸改性的介孔二氧化钛（记为 FeO@mTiO-MSA）组成的亲水性材料已被制备出来。研究表明，它是一种可行的糖肽和磷酸肽捕获吸附剂。该吸附剂结合了基于金属氧化物的亲和色谱和亲水作用液相色谱（HILIC）的特点，并利用了介孔二氧化钛的优点。使用磁性微球提供了磁响应并简化了分离。用 10%氨洗脱后，将肽进行 LC-MS/MS 分析。该方法仅使用 5μL 人唾液的三个分离重复样本，即可鉴定出 327 个磷酸肽和 65 个糖肽。其中，在 20 个肽段中检测到了磷酸化和糖基化位点。
