Whittle H C, Dossetor J, Oduloju A, Bryceson A D, Greenwood B M
J Clin Invest. 1978 Sep;62(3):678-84. doi: 10.1172/JCI109175.
Natural measles causes prolonged depression of cell-mediated immunity yet little is known as to how the infection influences lymphocyte function. Therefore, we studied the properties and function of lymphocytes during and after measles. The number and proportion of circulating thymus-derived lymphocytes was low during the acute stage of measles, and at this time 37% of these cells showed positive immunofluorescent staining for measles virus after stimulation with phytohemagglutinin. 7% of B cells were shown to contain virus but their numbers did not alter during the infection. Acute-phase lymphocytes, when stimulated, yielded infective virus and half were killed on incubation with autologous serum and complement. In acute measles the increase in [(3)H]-thymidine uptake of lymphocytes when stimulated with an optimal dose of PHA was normal in media with 10% fetal calf serum and low in media containing 10% autologous serum: the mean values were 56.8+/-34.1 and 23.7+/-25.9 cpm x 10(3) per 10(6) lymphocytes, respectively. Stimulation of acute-phase lymphocytes by Candida antigen was also low in media containing autologous serum averaging 1.2 x 10(3) cpm per 10(6) lymphocytes. On recovery 4-6 wk later this rose significantly to 18.9+/-19.8. The mean migration index of leukocytes to heat-killed candida cells in acute measles was 0.84+/-SD 0.08, and this fell significantly to 0.75+/-SD 0.08 4 wk later. Thus, depletion of T cells, an inhibitor of lymphocyte proliferation in the serum and a possible defect in antigen processing, interacts to depress cell-mediated immunity in measles.
自然感染麻疹会导致细胞介导免疫长期受到抑制，但对于这种感染如何影响淋巴细胞功能却知之甚少。因此，我们研究了麻疹期间及之后淋巴细胞的特性和功能。在麻疹急性期，循环中胸腺来源淋巴细胞的数量和比例较低，此时用植物血凝素刺激后，37%的这类细胞对麻疹病毒呈免疫荧光阳性染色。7%的B细胞被证明含有病毒，但它们的数量在感染期间没有改变。急性期淋巴细胞受到刺激时会产生感染性病毒，并且在与自体血清和补体孵育时半数会死亡。在急性麻疹中，用最佳剂量的PHA刺激时，淋巴细胞对[³H]胸腺嘧啶核苷的摄取在含有10%胎牛血清的培养基中正常，而在含有10%自体血清的培养基中较低：平均值分别为每10⁶个淋巴细胞56.8±34.1和23.7±25.9 cpm×10³。在含有自体血清的培养基中，白色念珠菌抗原对急性期淋巴细胞的刺激也较低，平均每10⁶个淋巴细胞为1.2×10³ cpm。4 - 6周后恢复时，这一数值显著上升至18.9±19.8。急性麻疹中白细胞对热灭活白色念珠菌细胞的平均迁移指数为0.84±标准差0.08，4周后显著降至0.75±标准差0.08。因此，T细胞的耗竭、血清中淋巴细胞增殖抑制剂以及抗原加工可能存在的缺陷相互作用，导致麻疹中细胞介导免疫受到抑制。
