Redox Regulation Laboratory, Department of Zoology, College of Basic Science and Humanities, Odisha University of Agriculture and Technology, Bhubaneswar, 751003, India.
Redox Regulation Laboratory, Department of Zoology, College of Basic Science and Humanities, Odisha University of Agriculture and Technology, Bhubaneswar, 751003, India.
Chemosphere. 2019 Jun;224:796-804. doi: 10.1016/j.chemosphere.2019.02.156. Epub 2019 Feb 26.
Rapidly expanding nanoparticle industries are predicted to have turnover of ∼$173.95 billion by 2025, indicating an urgency to study their comprehensive toxicological impact(s). Toxic effects of Graphene Oxide (GO) on oxidative stress physiology especially at mitochondrial level and redox modulation in fish in general and in climbing perch Anabas testudineus is absent. Therefore, we have investigated the toxic impacts of sub lethal doses of GO on selected oxidative stress physiology markers, protein and nucleic acid content along with haematological parameters in A. testudineus. Discriminant function and correlation analyses suggest that GO had toxic effects on the fish, as revealed from the studied parameters. Liver and gill tissues had shown strong response to GO than muscle. Augmented gradual accumulation of cellular lipid peroxides, specifically in mitochondria, was noticed. Activity of superoxide dismutase, catalase, and glutathione-S-transferase was augmented in contrast to the lowered level of the reduced glutathione titre. Alleviated total red blood corpuscle count and haemoglobin titre was parallel with an augmentation of white blood corpuscle count under GO administration. The protein level was also alleviated gradually in liver with clear changes in tissue specific nucleic acid levels, which was reduced under GO treatment. Results of the present study indicate that GO induces oxidative stress in cell and mitochondria in fish. Therefore, very careful future practices of use of GO directly, or as cargo in environmental monitoring processes in aquatic models in vitro in general and Pisces model in particular are suggested.
预计到 2025 年，快速发展的纳米颗粒产业的营业额将达到 1739.5 亿美元，这表明有必要研究其全面的毒理学影响。氧化石墨烯（GO）对鱼类特别是攀鲈的氧化应激生理学和线粒体水平以及氧化还原调节的毒性作用尚不清楚。因此，我们研究了亚致死剂量的 GO 对选定的氧化应激生理学标志物、蛋白质和核酸含量以及血液学参数的毒性影响。判别函数和相关分析表明，GO 对鱼类有毒性作用，从研究参数可以看出。肝脏和鳃组织对 GO 的反应比肌肉强。细胞脂质过氧化物，特别是在线粒体中的积累逐渐增加。超氧化物歧化酶、过氧化氢酶和谷胱甘肽-S-转移酶的活性增加，而还原型谷胱甘肽的水平降低。GO 给药后，总红细胞计数和血红蛋白水平降低，白细胞计数逐渐升高。肝组织中的蛋白质水平也逐渐降低，组织特异性核酸水平发生明显变化，GO 处理后降低。本研究结果表明，GO 诱导鱼类细胞和线粒体氧化应激。因此，建议在未来的实践中非常小心地使用 GO，无论是直接使用还是作为环境监测过程中的货物，特别是在水生模型和鱼类模型中。
