Patel College of Global Sustainability, University of South Florida, 4202 East Fowler Avenue, CGS 101, Tampa, FL 33620, USA.
Honors College and School of Geosciences, College of Arts and Sciences, University of South Florida, 4202 East Fowler Avenue, Tampa, FL 33620, USA.
Bioresour Technol. 2019 Jun;282:18-27. doi: 10.1016/j.biortech.2019.03.003. Epub 2019 Mar 2.
High water demand is a major challenge for the algae industry, so cultivating algae in wastewater can have the double benefit of biomass production and water remediation. The use of landfill leachate (LL), which is wastewater generated in landfills, was investigated to grow the microalga Picochlorum oculatum in a novel horizontal bioreactor (HBR), a low-cost modular cultivation system that reduces water evaporation and contamination risk thanks to its enclosed design. Pilot-scale (150 L) and commercial-scale (2000 L) HBRs that were operated outdoors in Florida using LL in batch and semi-continuous modes generated high cell density cultures (1.7·10 cells mL) and reached up to 1.9 g L of dry biomass suitable for biofuel production. Demonstrating the ability of ample non-potable water sources, such as LL, to support algae cultivation is essential for improving the sustainability and cost-effectiveness of commercial algal biofuels and bioproducts, as freshwater resources become increasingly scarce.
高水需求是藻类产业的主要挑战，因此在废水中培养藻类具有生物量生产和水修复的双重效益。研究了利用垃圾渗滤液（LL）——垃圾填埋场产生的废水——来在新型水平生物反应器（HBR）中培养微藻眼斑拟微绿球藻。HBR 是一种低成本的模块化培养系统，由于其封闭设计，可减少水蒸发和污染风险。在佛罗里达州，使用 LL 进行批处理和半连续模式在户外运行的中试规模（150L）和商业规模（2000L）HBR 产生了高细胞密度培养物（1.7×10 个细胞/mL），并达到了 1.9g/L 的干生物量，适合生物燃料生产。证明充足的非饮用水源（如 LL）能够支持藻类培养对于提高商业藻类生物燃料和生物制品的可持续性和成本效益至关重要，因为淡水资源变得越来越稀缺。
