Dritsas Panagiotis, Aggelis George
Department of Biology, School of Natural Sciences, University of Patras, 26500 Patras, Greece.
Microorganisms. 2025 May 18;13(5):1155. doi: 10.3390/microorganisms13051155.
The microalgal strains VAS2.5, SAG4.4, PAT3.2B, VON5.3, and PAT2.7 were cultured in a Stirred Tank Reactor at 25 °C or 20 °C in modified artificial seawater and their biotechnological potential was assessed. VAS2.5, VON5.3, and PAT2.7 were high in biomass production at both temperatures (i.e., 438.8-671.3 mg/L and 418.4-546.7 mg/L at 25 °C and 20 °C, respectively), though strains grew only at 25 °C. The highest lipid percentage was recorded for the cultures of VAS2.5 (19.3 ± 0.7%) and VON5.3 (16.4 ± 1.5%) at 25 °C, notably rich in C20:5, while PAT2.7 proved a major producer of C16:1. The predominant lipid fraction was glycolipids and sphingolipids (41.3-57.4%) for VAS2.5, PAT2.7 at 25 °C and VON5.3 at 20 °C and neutral lipids (55.6-63.5%) in the other cultures, indicating the different effect of temperature on lipid synthesis of the various microalgae. Additionally, almost all strains stood out for their high protein content, exceeding 50% in the culture of PAT3.2B, but polysaccharide and pigment content were not high. The biochemical profiles of the isolates showcased their suitability for use primarily as feed additives in the aquaculture sector.
微藻菌株VAS2.5、SAG4.4、PAT3.2B、VON5.3和PAT2.7在搅拌釜式反应器中于25℃或20℃的改良人工海水中培养，并评估了它们的生物技术潜力。VAS2.5、VON5.3和PAT2.7在这两个温度下的生物量产量都很高（即25℃和20℃时分别为438.8 - 671.3 mg/L和418.4 - 546.7 mg/L），尽管SAG4.4仅在25℃生长。VAS2.5（19.3±0.7%）和VON5.3（16.4±1.5%）在25℃培养时脂质百分比最高，富含C20:5，而PAT2.7是C16:1的主要生产者。VAS2.5、25℃下的PAT2.7和20℃下的VON5.3的主要脂质部分是糖脂和鞘脂（41.3 - 57.4%），其他培养物中的主要脂质部分是中性脂质（55.6 - 63.5%），这表明温度对不同微藻脂质合成的影响不同。此外，几乎所有菌株的蛋白质含量都很高，PAT3.2B培养物中的蛋白质含量超过50%，但多糖和色素含量不高。分离物的生化特征表明它们主要适合用作水产养殖部门的饲料添加剂。
