School of Materials Science and Engineering, Ocean University of China, 266100, Qingdao, PR China.
School of Materials Science and Engineering, Ocean University of China, 266100, Qingdao, PR China.
Talanta. 2024 Aug 15;276:126234. doi: 10.1016/j.talanta.2024.126234. Epub 2024 May 14.
Glutathione (GSH) is an essential antioxidant in the human body, but its detection is difficult due to the interference of complex components in serum. Herein, hollow double-layer Pt@CeO nanospheres were developed as oxidase mimetics, and the light-assisted oxidase mimetics effects were found. The oxidase activity was enhanced significantly by utilizing the synergistic effect of Schottky junction and the localized surface plasmon resonance (LSPR) of Pt under UV light. A novel GSH colorimetric-fluorescent-SERS sensing platform was established, with the sensing performance notably boosted by using the light-assisted oxidase mimetics effects. This platform boasts an exceptionally low detection limit (LOD) of 0.084 μM, while the detection time was shortened from 10 min to just 2 min. The anti-interference detection with high recovery rate (96.84%-107.4 %) in real serum made it be promising for practical application.
谷胱甘肽（GSH）是人体内一种必需的抗氧化剂，但由于血清中复杂成分的干扰，其检测较为困难。在此，我们开发了中空双层 Pt@CeO 纳米球作为氧化酶模拟物，并发现了光辅助氧化酶模拟物效应。在紫外光下，利用肖特基结和 Pt 的局域表面等离子体共振（LSPR）的协同作用，显著增强了氧化酶的活性。建立了一种新型的 GSH 比色-荧光-SERS 传感平台，通过利用光辅助氧化酶模拟物效应，显著提高了传感性能。该平台具有极低的检测限（LOD）为 0.084 μM，而检测时间从 10 分钟缩短至仅 2 分钟。在实际血清中具有高回收率（96.84%-107.4%）的抗干扰检测使其具有实际应用的潜力。
