鞘内注射曲安奈德对大鼠实验性自身免疫性神经炎具有抗炎作用，并对施万细胞具有直接抗氧化作用。

Intrathecal triamcinolone acetonide exerts anti-inflammatory effects on Lewis rat experimental autoimmune neuritis and direct anti-oxidative effects on Schwann cells.

机构信息

Department of Neurology, St. Josef Hospital, Ruhr-University Bochum, Gudrunstr. 56, 44791, Bochum, Germany.

出版信息

J Neuroinflammation. 2019 Mar 9;16(1):58. doi: 10.1186/s12974-019-1445-0.

BACKGROUND

Corticosteroids dominate in the treatment of chronic autoimmune neuropathies although long-term use is characterized by devastating side effects.

METHODS

We introduce the intrathecal application of the synthetic steroid triamcinolone (TRIAM) as a novel therapeutic option in experimental autoimmune neuritis in Lewis rats RESULTS: After immunization with neuritogenic P2 peptide, we show a dose-dependent therapeutic effect of one intrathecal injection of 0.3 or 0.6 mg/kg TRIAM on clinical and electrophysiological parameters of neuritis with a lower degree of inflammatory infiltrates (T cells and macrophages) and demyelination in the sciatic nerve. In vitro studies in Schwann cell cultures showed an increased expression of IL-1 receptor antagonist and reduced expression of Toll-like receptor 4 after incubation with TRIAM as well as a protective effect of TRIAM against oxidative stress after HO exposure.

CONCLUSION

Intrathecal TRIAM application could be a novel immunomodulatory and potentially neuroprotective option for autoimmune neuropathies with a direct effect on Schwann cells.

背景

尽管长期使用皮质类固醇会产生破坏性的副作用，但它们在治疗慢性自身免疫性神经病中占主导地位。

方法

我们介绍了鞘内应用合成类固醇曲安奈德（TRIAM）作为实验性自身免疫性神经炎中Lewis 大鼠的一种新的治疗选择。

结果

在用致神经炎 P2 肽免疫后，我们发现鞘内注射 0.3 或 0.6mg/kg TRIAM 对神经炎的临床和电生理参数具有剂量依赖性的治疗作用，坐骨神经中的炎症浸润（T 细胞和巨噬细胞）和脱髓鞘程度较低。在雪旺细胞培养物中的体外研究表明，TRIAM 孵育后白细胞介素 1 受体拮抗剂表达增加，Toll 样受体 4 表达减少，HO 暴露后 TRIAM 具有保护作用。