Department of Hepatobiliary Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
Faculty of Materials Science & Engineering, Hubei University, Wuhan, 430062, China.
Anal Chim Acta. 2019 Jun 13;1058:80-88. doi: 10.1016/j.aca.2019.01.031. Epub 2019 Jan 25.
Herein, graphite nanosheets (GN) were first prepared through simple liquid-phase exfoliation of graphite powder in N, N-dimethylacetamide (DMAC). After then, ultrasmall Cu-based metal organic frame (HKUST-1) nanoparticles (less than 5 nm) were in-situ anchored on the surface of graphite nanosheets with high degree of dispersion. Due to the intrinsic structural advantages of the as-synthesized HKUST-1 nanoparticles decorated graphite nanosheets (HKUST-1/GN) hybrids, including superior electron transfer ability and the greatly enhanced adsorption property, HKUST-1/GN shows excellent electrochemical sensing performance toward DNA damage biomarker 8-hydroxy-2'-deoxyguanosine with fast detection speed (∼240 s), wide linear window (10 nM-1 μM), high sensitivity (346857 μA mM cm), low detection limit (∼2.5 nM), and good reproducibility. As a result, a highly sensitive electrochemical sensing platform for the detection of DNA damage biomarker 8-hydroxy-2'-deoxyguanosine was fabricated basing the as-prepared HKUST-1/GN hybrids. What is more, the developed electrochemical method was successfully used for the detection of real samples and exhibited satisfied result.
在此，通过在 N，N-二甲基乙酰胺（DMAC）中简单的液相剥离石墨粉末，首次制备了石墨纳米片（GN）。然后，将超小的基于铜的金属有机框架（HKUST-1）纳米颗粒（小于 5nm）原位锚定在具有高分散度的石墨纳米片表面上。由于所合成的 HKUST-1 纳米颗粒修饰的石墨纳米片（HKUST-1/GN）杂化物具有固有结构优势，包括卓越的电子转移能力和大大增强的吸附性能，HKUST-1/GN 对 DNA 损伤生物标志物 8-羟基-2'-脱氧鸟苷显示出优异的电化学传感性能，具有快速的检测速度（约 240s）、宽线性窗口（10nM-1μM）、高灵敏度（346857μA·mM·cm）、低检测限（约 2.5nM）和良好的重现性。因此，基于所制备的 HKUST-1/GN 杂化物构建了用于检测 DNA 损伤生物标志物 8-羟基-2'-脱氧鸟苷的高灵敏度电化学传感平台。此外，所开发的电化学方法已成功用于实际样品的检测，并取得了令人满意的结果。
