School of Pharmaceutical Sciences, Tsinghua University, Beijing, 100084, China; Tsinghua-Peking Joint Center for Life Sciences, Beijing, 100084, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, China.
Material Institute, Beijing Institute of Technology, Beijing, 100081, China.
Anal Chim Acta. 2019 Jun 13;1058:89-96. doi: 10.1016/j.aca.2019.01.036. Epub 2019 Jan 29.
Paper based biosensor devices have the benefit of easy-use, low-cost and environmental friendly for point-of-care diagnostic application. In this research, we report a novel form of paper based biosensor with hierarchical assembled nanomaterials and MOF enhanced bio-probes for the simultaneous detection of microRNA-141 (miR-141) and microRNA-21 (miR-21) which have the potential for early cancer detection. The combination of multi-dimensional nanomaterial allows low impedance and high sensing area on typical poorly defined paper substrate. MOF conjugated bio-probe, methylene blue (MB) and ferrocene (Fc) with distinguishable electrochemical signal, further contributed to the high selectivity and sensitivity. Simultaneously detection of miR-141 and miR-21 with a detection limit of 0.1 fM was demonstrated under optimal conditions. All these features enable accurately target detection in serum samples. Therefore, this strategy is of promising as an inexpensive, easy access diagnosis platform for cancers by means of simultaneously detection of a variety of miRNA biomarkers.
基于纸张的生物传感器设备具有易于使用、低成本和环保的优势，非常适用于即时诊断应用。在这项研究中，我们报告了一种新颖的基于纸张的生物传感器形式，它采用分层组装的纳米材料和 MOF 增强的生物探针，用于同时检测 microRNA-141 (miR-141) 和 microRNA-21 (miR-21)，这两种 microRNA 具有早期癌症检测的潜力。多种纳米材料的组合允许在典型的定义不明确的纸张基底上实现低阻抗和高传感面积。与具有可区分电化学信号的甲亚胺蓝 (MB) 和二茂铁 (Fc) 结合的 MOF 缀合生物探针进一步提高了选择性和灵敏度。在最佳条件下，实现了对 miR-141 和 miR-21 的同时检测，检测限低至 0.1 fM。所有这些特性都能够实现血清样本中目标物的精确检测。因此，这种策略有望成为一种通过同时检测多种 miRNA 生物标志物来进行癌症低成本、便捷诊断的平台。
