Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Biosens Bioelectron. 2019 Apr 15;131:299-306. doi: 10.1016/j.bios.2019.02.029. Epub 2019 Feb 19.
A sandwich-type photoelectrochemical (PEC) immunosensing platform was designed for detection of amino-terminal pro-B-type natriuretic peptide (NT-pro BNP). Thereinto, flower-like BiWO/AgS nanoparticles (F-BiWO/AgS) were employed as photoelectrochemical matrix, and graphene oxide and polydopamine composite (GO/PDA) were prepared as signal labels. In this proposal, AgS was in-situ growth on the surface of F-BiWO modified with thioglycolic acid (TGA). Specially, a cascade-like band-edge level between F-BiWO and AgS effectively improved the photocurrent conversion efficiency and enhanced the photocurrent response. Then, the conjugated GO/PDA aimed to further amplify signal because PDA as electron donor could sweep the holes and inhibit the recombination of photogenerated electron-hole pairs, while GO owned brilliant conductivity speeding up the electrons transfer. The photocurrent increased with the amount of GO/PDA conjugates which had positive correlation with the NT-pro BNP. Under optimal experimental conditions, the proposed sandwich-type PEC immunosensor presented a desirable linear relationship ranged from 0.1 pg/mL to 100 ng/mL for NT-pro BNP with the detection limit of 0.03 pg/mL (S/N = 3). The prepared PEC immunosensor exhibited high stability and selectivity, which offered an innovative idea for the detection of other biomolecules.
设计了一种三明治型光电化学（PEC）免疫传感平台，用于检测氨基末端 pro-B 型利钠肽（NT-pro BNP）。其中，花状 BiWO/AgS 纳米粒子（F-BiWO/AgS）用作光电化学基质，氧化石墨烯和聚多巴胺复合物（GO/PDA）用作信号标记。在本方案中，AgS 原位生长在经巯基乙酸（TGA）修饰的 F-BiWO 表面。特别地，F-BiWO 和 AgS 之间的级联能带结构有效提高了光电流转换效率并增强了光电流响应。然后，共轭的 GO/PDA 旨在进一步放大信号，因为 PDA 作为电子供体能扫除空穴并抑制光生电子-空穴对的复合，而 GO 则具有出色的导电性，能加速电子转移。光电流随着 GO/PDA 结合物的量增加而增加，这与 NT-pro BNP 呈正相关。在最佳实验条件下，所提出的三明治型 PEC 免疫传感器对 NT-pro BNP 表现出从 0.1 pg/mL 到 100 ng/mL 的良好线性关系，检测限为 0.03 pg/mL（S/N = 3）。该制备的 PEC 免疫传感器表现出高稳定性和选择性，为其他生物分子的检测提供了新的思路。
