Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China.
Hand and Foot Surgery Department, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, China.
Anal Chim Acta. 2021 Dec 15;1188:339190. doi: 10.1016/j.aca.2021.339190. Epub 2021 Oct 19.
In this paper, we constructed a sandwich-type photoelectrochemical (PEC) immunosensor for the quantitative detection of procalcitonin (PCT) based on the sensitization of Mn doped CdS (CdS:Mn) nanocomposites to BiWO and the signal amplification effect of an upconversion material NaYF:Yb,Tm. BiWO was synthesized with a three-dimensional flowered structure. CdS:Mn reduced the recombination of photogenerated carriers, and significantly improved photocurrent response. Lanthanide-doped upconversion nanomaterials were used as the label of secondary antibody. NaYF:Yb,Tm have two functions, not only connected with the secondary antibody, but also can further amplify the photocurrent response. The proposed immunosensor for detecting PCT provided a desired linear range of 0.5 pg mL-100 ng mL and a detection limit of 0.13 pg mL under optimal experimental conditions. Besides, the PEC immunosensor demonstrated good reproducibility, specificity and stability. The results of determination of PCT in real human serum samples were satisfactory. Thus, the immunosensor may be applied in the clinical diagnosis of PCT and other biomarkers.
本文构建了一种基于 Mn 掺杂 CdS(CdS:Mn)纳米复合材料敏化 BiWO 和上转换材料 NaYF:Yb,Tm 的信号放大效应的三明治型光电化学(PEC)免疫传感器，用于降钙素原(PCT)的定量检测。采用三维花状结构合成了 BiWO。CdS:Mn 减少了光生载流子的复合，显著提高了光电流响应。镧系掺杂上转换纳米材料被用作次级抗体的标记物。NaYF:Yb,Tm 具有两种功能，不仅与次级抗体相连，而且可以进一步放大光电流响应。在最佳实验条件下，用于检测 PCT 的所提出的 PEC 免疫传感器提供了期望的线性范围为 0.5 pg mL-100 ng mL 和检测限为 0.13 pg mL。此外，PEC 免疫传感器表现出良好的重现性、特异性和稳定性。在实际人血清样本中测定 PCT 的结果令人满意。因此，该免疫传感器可应用于 PCT 和其他生物标志物的临床诊断。
