Department of Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
National Center for Agricultural Utilization Research, USDA, Peoria, IL, USA.
Bioresour Technol. 2019 Jun;282:103-109. doi: 10.1016/j.biortech.2019.02.123. Epub 2019 Mar 1.
Corn stover and sugarcane bagasse are the most widely available agriculture processing biomass and could serve as feedstocks for production of biofuel. In this study, three different technologies are combined to develop a more efficient conversion process for each of these feedstocks. The three technologies are diluted alkaline deacetylation process, combined thermochemical and mechanical shear pretreatment, and fermentation using a combined inoculum of two commercial Saccharomyces yeast strains. The two yeast strains used were a non-GMO and GMO strain engineered for xylose fermentation. The final ethanol concentrations obtained were 35.7 g/L from deacetylated corn stover and 32.9 g/L from sugarcane bagasse. Blending the two yeast reduced residual xylose content from 1.24 g/L to 0.48 g/L and increased ethanol production by 6.5% compared to solely using the C5/C6 yeast. The optimized yeast blend also lowered the amount of C5/C6 yeast required for inoculation by 80%.
玉米秸秆和甘蔗渣是最广泛可用的农业加工生物质，可以作为生物燃料生产的原料。在这项研究中，三种不同的技术结合起来，为这两种原料开发了一种更有效的转化工艺。这三种技术是稀堿性去乙酰化工艺、热化学和机械剪切联合预处理以及使用两种商业酿酒酵母混合接种物发酵。所使用的两种酵母菌株是经过基因改造用于木糖发酵的非转基因和转基因菌株。从去乙酰化玉米秸秆中获得的最终乙醇浓度为 35.7g/L，从甘蔗渣中获得的乙醇浓度为 32.9g/L。与仅使用 C5/C6 酵母相比，混合使用两种酵母将残余木糖含量从 1.24g/L 降低到 0.48g/L，乙醇产量提高了 6.5%。优化后的酵母混合物还将接种所需的 C5/C6 酵母量降低了 80%。
