School of Food Science and Biotechnology, Kyungpook National University, Daegu, 41566, South Korea.
Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, South Korea.
Appl Microbiol Biotechnol. 2020 Apr;104(8):3245-3252. doi: 10.1007/s00253-020-10427-z. Epub 2020 Feb 19.
With growing interest in alternative fuels to minimize carbon and particle emissions, research continues on the production of lignocellulosic ethanol and on the development of suitable yeast strains. However, great diversities and continued technical advances in pretreatment methods for lignocellulosic biomass complicate the evaluation of developed yeast strains, and strain development often lags industrial applicability. In this review, recent studies demonstrating developed yeast strains with lignocellulosic biomass hydrolysates are compared. For the pretreatment methods, we highlight hydrothermal pretreatments (dilute acid treatment and autohydrolysis), which are the most commonly used and effective methods for lignocellulosic biomass pretreatment. Rather than pretreatment conditions, the type of biomass most strongly influences the composition of the hydrolysates. Metabolic engineering strategies for yeast strain development, the choice of xylose-metabolic pathway, adaptive evolution, and strain background are highlighted as important factors affecting ethanol yield and productivity from lignocellulosic biomass hydrolysates. A comparison of the parameters from recent studies demonstrating lignocellulosic ethanol production provides useful information for future strain development.
随着人们对替代燃料的兴趣日益浓厚，以尽量减少碳和颗粒排放，关于木质纤维素乙醇的生产和合适酵母菌株的开发的研究仍在继续。然而，木质纤维素生物质预处理方法的多样性和不断的技术进步使得开发的酵母菌株的评估变得复杂，而且菌株的开发往往落后于工业适用性。在这篇综述中，比较了最近展示用木质纤维素生物质水解物培养的酵母菌株的研究。对于预处理方法，我们重点介绍水热预处理（稀酸处理和自水解），这是最常用和有效的木质纤维素生物质预处理方法。影响水解物成分的不是预处理条件，而是生物质的类型。酵母菌株开发的代谢工程策略、木糖代谢途径的选择、适应性进化和菌株背景被强调为影响木质纤维素生物质水解物乙醇产率和生产力的重要因素。对最近展示木质纤维素乙醇生产的研究的参数进行比较，为未来的菌株开发提供了有用的信息。
