Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland; Faculty of Science, University of Basel, Mattenstrasse 26, CH-4058 Basel, Switzerland.
Curr Opin Biotechnol. 2019 Oct;59:31-38. doi: 10.1016/j.copbio.2019.02.003. Epub 2019 Mar 7.
Synthetic biology uses engineering principles to design and assemble biological components and systems for a variety of applications. On the basis of genetic engineering, synthetic gene switches can be interconnected to construct complex gene circuits, capable of sensing and integrating diverse input signals for precise spatiotemporal control of target gene expression in living cells. Designer cells can be equipped with advanced gene circuitry enabling them to react precisely to pre-programmed combinations of conditions, automatically triggering a specified response, such as therapeutic protein production. Such cells are promising therapeutic modalities for applications where traditional medical treatments have limitations. Herein, we highlight selected recent examples of designer cells with engineered gene circuits targeted toward applications in personalized human medicine.
合成生物学利用工程原理设计和组装生物组件和系统，以实现各种应用。在基因工程的基础上，合成基因开关可以相互连接，构建复杂的基因电路，能够感知和整合多样化的输入信号，从而实现对活细胞中靶基因表达的精确时空控制。设计细胞可以配备先进的基因电路，使其能够精确地对预先编程的条件组合做出反应，自动触发特定的响应，如治疗性蛋白质的产生。在传统医学治疗方法存在局限性的应用中，这些细胞是很有前途的治疗模式。在此，我们重点介绍了一些最近设计的带有工程基因电路的细胞，这些细胞的目标是应用于个性化的人类医学。
