Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
Occup Environ Med. 2019 Jul;76(7):482-487. doi: 10.1136/oemed-2018-105537. Epub 2019 Mar 9.
To investigate the relation between signs and symptoms of irritation and biomarkers of inflammatory markers in blood in healthy volunteers exposed to different chemical vapours for 2 or 4 hours in an exposure chamber.
The investigated chemicals were: acetic acid (5 and 10 ppm), acrolein (0.05 and 0.1 ppm), 1,4-dioxane (20 ppm), n-hexanal (2 and 10 ppm), hydrogen peroxide (0.5 and 2.2 ppm), 2-propanol (150 ppm), m-xylene (50 ppm), standard and dearomatised white spirit (100 and 300 mg/m). C reactive protein (CRP), serum amyloid A protein and interleukin 6 were measured in plasma immediately before and 2 or 4 hours after the exposures. Symptoms were rated from 0 to 100 mm in Visual Analogue Scales and covered 10 questions whereof four related to irritation: discomfort in the eyes, nose and throat and dyspnoea. The effect measurements included blink frequency by electromyography, nasal swelling by acoustic rhinometry and lung function by spirometry.
Logistic quantile regression analyses revealed no significant associations except a negative relation between ratings of irritation and CRP.
The results suggest a down-regulation of CRP after short-term exposure to low levels of vapours of irritating chemicals. This response might be mediated by the cholinergic anti-inflammatory pathway and further studies are recommended in order to refute or confirm this hypothesis.
在暴露室内，将健康志愿者暴露于不同化学蒸气中 2 或 4 小时，以调查刺激的体征和症状与血液中炎症标志物生物标志物之间的关系。
研究的化学物质有：乙酸（5 和 10 ppm）、丙烯醛（0.05 和 0.1 ppm）、1,4-二恶烷（20 ppm）、正己醛（2 和 10 ppm）、过氧化氢（0.5 和 2.2 ppm）、2-丙醇（150 ppm）、间二甲苯（50 ppm）、标准和脱芳烃白油（100 和 300 mg/m）。在暴露前后立即测量血浆中的 C 反应蛋白（CRP）、血清淀粉样蛋白 A 蛋白和白细胞介素 6。症状通过视觉模拟量表从 0 到 100 毫米进行评分，涵盖了 10 个问题，其中 4 个与刺激有关：眼睛、鼻子和喉咙不适以及呼吸困难。效应测量包括肌电图的眨眼频率、声反射鼻测量的鼻肿胀和肺活量计的肺功能。
逻辑分位数回归分析显示，除了刺激评分与 CRP 之间的负相关外，没有发现显著关联。
结果表明，在短时间暴露于低水平刺激化学蒸气后，CRP 下调。这种反应可能是由胆碱能抗炎途径介导的，建议进一步研究以反驳或证实这一假设。
