管胶法：一种用于高通量定量蛋白质组学的快速有效样品制备方法。

Tube-Gel: A Fast and Effective Sample Preparation Method for High-Throughput Quantitative Proteomics.

作者信息

Muller Leslie, Fornecker Luc, Cianferani Sarah, Carapito Christine

机构信息

Laboratoire de Spectrométrie de Masse Bio-Organique (LSMBO), IPHC, UMR 7178, Université de Strasbourg, CNRS, Strasbourg, France.

出版信息

Methods Mol Biol. 2019;1959:123-127. doi: 10.1007/978-1-4939-9164-8_8.

DOI:10.1007/978-1-4939-9164-8_8

PMID:30852819

Abstract

Sample preparation is a key step in proteomics workflows. Tube-gel (TG) is a fast and repeatable sample preparation method that consists in the instantaneous trapping of the sample in a polyacrylamide gel matrix. It takes advantage of in-gel sample preparations by allowing the use of high concentrations of sodium-dodecyl sulfate but avoids the time-consuming step of electrophoresis. Therefore, TG limits the sample handling and is thus particularly suitable for high-throughput quantitative proteomics when large sample numbers have to be processed, as it is often the case in biomarker research and clinical proteomics projects.

摘要

样品制备是蛋白质组学工作流程中的关键步骤。管凝胶（TG）是一种快速且可重复的样品制备方法，其原理是将样品瞬间捕获在聚丙烯酰胺凝胶基质中。它利用凝胶内样品制备方法，允许使用高浓度的十二烷基硫酸钠，但避免了耗时的电泳步骤。因此，TG减少了样品处理，特别适用于需要处理大量样品的高通量定量蛋白质组学，在生物标志物研究和临床蛋白质组学项目中经常如此。