使用自上而下质谱法和诊断离子检测蛋白质变体
Detection of Proteoforms Using Top-Down Mass Spectrometry and Diagnostic Ions.
作者信息
Coelho Graça Didia, Hartmer Ralf, Jabs Wolfgang, Scherl Alexander, Clerici Lorella, Samii Kaveh, Tsybin Yury O, Hochstrasser Denis, Lescuyer Pierre
机构信息
Clinical Proteomics and Chemistry Group, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
Department of Genetic, Laboratory Medicine and Pathology, Geneva University Hospitals, Geneva, Switzerland.
出版信息
Methods Mol Biol. 2019;1959:173-183. doi: 10.1007/978-1-4939-9164-8_12.
Characterization of protein structure modifications is an important field in mass spectrometry (MS)-based proteomics. Here, we describe a process to quickly and reliably identify a mass change in a targeted protein sequence by top-down mass spectrometry (TD MS) using electron transfer dissociation (ETD). The step-by-step procedure describes how to develop a TD MS method for data acquisition as well as the data analysis process. The described TD MS workflow utilizes diagnostic ions to characterize an unknown sample in a few hours.
蛋白质结构修饰的表征是基于质谱（MS）的蛋白质组学中的一个重要领域。在此，我们描述了一种通过使用电子转移解离（ETD）的自上而下质谱（TD MS）快速可靠地识别目标蛋白质序列中质量变化的方法。该分步程序描述了如何开发用于数据采集的TD MS方法以及数据分析过程。所描述的TD MS工作流程利用诊断离子在几小时内对未知样品进行表征。
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