Ekicier Acar Selcan, Sarıcaoğlu M Sinan, Çolak Aysel, Aktaş Zeynep, Sepici Dinçel Aylin
Eye Clinic, Ankara Kecioren Training and Research Hospital, Ankara, Turkey.
Eye Clinic, Ankara Numune Training and Research Hospital, Ankara, Turkey.
Eur J Ophthalmol. 2020 Jul;30(4):714-722. doi: 10.1177/1120672119833271. Epub 2019 Mar 11.
We aimed to create mechanic optic nerve injury model in rats and investigate the neuroprotective effects of topical Coenzyme Q10 + Vitamin E (CoQ + Vit.E) molecules on retinal ganglion cells.
Mechanic optic nerve injury model was created in the right eyes of rats (n = 12). Rats were divided into two groups: glaucoma model with sham treatment and topical CoQ + Vit.E treatment. Treatment was applied for 4 weeks. Glial fibrillary acidic protein, Brn-3a antibody, and anti-Iba1 were examined by immunohistochemistry. Glial fibrillary acidic protein, Bax, Bcl-xL, and Tfam protein expression were measured by Western blot analysis.
The number of Brn-3a-positive retinal ganglion cell was 15.0 ± 1.0 (min: 14, max: 16) in sham treatment group and 22.2 ± 4.8 (min: 18, max: 29) in topical CoQ10 + Vit.E treatment group. The protection of Brn-3a in CoQ10 + Vit.E was statistically significant (p < 0.05). Glial fibrillary acidic protein-positive astroglial counts were recorded as 11.7 ± 2.1 (min: 10, max: 14) in sham treatment and 2.5 ± 1.5 (min: 1, max: 4) in topical CoQ10 + Vit.E treatment group (p < 0.05). Topical CoQ10 + Vit.E treatment also decreased Iba1 expression in the retina of mechanic optic nerve injury groups. CoQ10 + Vit.E treatment prevented apoptotic cell death by increasing Bcl-xL protein expression. Also, CoQ10 + Vit.E preserved Tfam protein expression in the retina.
This study has shown that in glaucoma treatment the neuron protecting effect of topical CoQ10 + Vit.E molecules can be valuable.
我们旨在建立大鼠机械性视神经损伤模型，并研究局部应用辅酶Q10 + 维生素E（CoQ + Vit.E）分子对视网膜神经节细胞的神经保护作用。
在大鼠（n = 12）的右眼建立机械性视神经损伤模型。大鼠分为两组：假治疗青光眼模型组和局部应用CoQ + Vit.E治疗组。治疗持续4周。通过免疫组织化学检测胶质纤维酸性蛋白、Brn-3a抗体和抗Iba1。通过蛋白质印迹分析测量胶质纤维酸性蛋白、Bax、Bcl-xL和Tfam蛋白表达。
假治疗组中Brn-3a阳性视网膜神经节细胞数量为15.0 ± 1.0（最小值：14，最大值：16），局部应用CoQ10 + Vit.E治疗组为22.2 ± 4.8（最小值：18，最大值：29）。CoQ10 + Vit.E对Brn-3a的保护作用具有统计学意义（p < 0.05）。假治疗组中胶质纤维酸性蛋白阳性星形胶质细胞计数为11.7 ± 2.1（最小值：10，最大值：14），局部应用CoQ10 + Vit.E治疗组为2.5 ± 1.5（最小值：1，最大值：4）（p < 0.05）。局部应用CoQ10 + Vit.E治疗还降低了机械性视神经损伤组视网膜中Iba1的表达。CoQ10 + Vit.E治疗通过增加Bcl-xL蛋白表达预防凋亡性细胞死亡。此外，CoQ10 + Vit.E保留了视网膜中Tfam蛋白表达。
本研究表明，在青光眼治疗中，局部应用CoQ10 + Vit.E分子的神经保护作用可能具有重要价值。
