Filigenzi Michael S, Graves Emily E, Tell Lisa A, Jelks Karen A, Poppenga Robert H
California Animal Health and Food Safety Laboratory (Filigenzi, Poppenga).
Department of Environmental Science and Policy (Graves).
J Vet Diagn Invest. 2019 May;31(3):399-407. doi: 10.1177/1040638719834329. Epub 2019 Mar 11.
We developed and validated a liquid chromatography-high-resolution mass spectrometry (LC-HRMS) analytical method for quantitatively measuring pesticide concentrations in small-body avian tissue samples using homogenized 1-2-d-old chicken carcasses as the test matrix. We quantified the following key insecticides: sulfoxaflor (sulfoximine class) and the neonicotinoids dinotefuran, nitenpyram, thiamethoxam, acetamiprid, thiacloprid, clothianidin, and imidacloprid. We used fortified chick carcass samples to validate method accuracy (80-125% recoveries), precision (<20% relative standard deviation), and sensitivity (≤1.2 ppb) for these targeted analytes. This method also uses full-scan, high-resolution MS to screen for the presence of a wide variety of other xenobiotics in bird carcasses. The utility of our screening process was demonstrated by the detection of carbaryl in some samples. This sensitive LC-HRMS analytical method for insecticide detection in a matrix of homogenized carcass is ideal for evaluating small birds for insecticide exposure. This novel whole-carcass method may allow for research studies of small-bodied, free-ranging avian species, and could provide insight regarding their exposure to multiple classes of environmental contaminants.
我们开发并验证了一种液相色谱-高分辨率质谱（LC-HRMS）分析方法，该方法使用1至2日龄鸡的匀浆尸体作为测试基质，定量测量小型鸟类组织样本中的农药浓度。我们对以下关键杀虫剂进行了定量分析：氟啶虫胺腈（砜亚胺类）以及新烟碱类杀虫剂呋虫胺、烯啶虫胺、噻虫嗪、啶虫脒、噻虫啉、氯噻啉和吡虫啉。我们使用添加了目标分析物的鸡尸体样本验证了该方法的准确性（回收率80-125%）、精密度（相对标准偏差<20%）和灵敏度（≤1.2 ppb）。该方法还使用全扫描、高分辨率质谱来筛查鸟类尸体中多种其他外源化合物的存在。在一些样本中检测到西维因，证明了我们筛查过程的实用性。这种用于在匀浆尸体基质中检测杀虫剂的灵敏LC-HRMS分析方法，非常适合评估小型鸟类的杀虫剂暴露情况。这种全新的全尸体方法可能有助于对小型自由放养鸟类物种进行研究，并能提供有关它们接触多种环境污染物的见解。
