Price R A, Ogston D, Macdonald A G
Undersea Biomed Res. 1986 Mar;13(1):63-75.
An apparatus which enables aggregating agents to be added to a stirred suspension of platelets subjected to a pressure of up to 600 ATA is described. The ensuing aggregation reaction is monitored optically. The pressure is applied with helium; data are presented that indicate the effects are mainly attributable to hydrostatic pressure. The aggregation of human platelets induced by ADP was reversibly inhibited by pressure in the 1-270 ATA range. The second phase of aggregation was blocked by 65 ATA; the rate of first phase aggregation was reduced but the time to respond to ADP was not affected. Comparable pressures also affected collagen and arachidonate-induced aggregation. The time to respond to these aggregating agents was prolonged and both the rate and the degree of aggregation were reduced by pressure.
描述了一种装置，该装置能够将聚集剂添加到承受高达600ATA压力的搅拌血小板悬浮液中。随后的聚集反应通过光学监测。压力由氦气施加；所呈现的数据表明这些效应主要归因于静水压力。在1 - 270ATA范围内，压力可逆转地抑制由ADP诱导的人血小板聚集。聚集的第二阶段在65ATA时被阻断；第一阶段聚集的速率降低，但对ADP作出反应的时间不受影响。类似的压力也影响胶原蛋白和花生四烯酸诱导的聚集。对这些聚集剂作出反应的时间延长，并且聚集的速率和程度都因压力而降低。
