Bai Lijun, Chen Qing, Jiang Leiyu, Lin Yuanxiu, Ye Yuntian, Liu Peng, Wang Xiaorong, Tang Haoru
1College of Horticulture, Sichuan Agricultural University, Chengdu, 611130 China.
Chengdu Life Baseline Technology Co., LTD, Chengdu, China.
Hortic Res. 2019 Mar 4;6:42. doi: 10.1038/s41438-019-0128-4. eCollection 2019.
To investigate the molecular mechanism underlying fruit development and color change, comparative transcriptome analysis was employed to generate transcriptome profiles of two typical wild varieties of at three fruit developmental stages (green fruit stage, turning stage, and ripe fruit stage). We identified 25,699 long noncoding RNAs (lncRNAs) derived from 25,107 loci in the fruit transcriptome, which showed distinct stage- and genotype-specific expression patterns. Time course analysis detected a large number of differentially expressed protein-coding genes and lncRNAs associated with fruit development and ripening in both of the varieties. The target genes downregulated in the late stages were enriched in terms of photosynthesis and cell wall organization or biogenesis, suggesting that lncRNAs may act as negative regulators to suppress photosynthesis and cell wall organization or biogenesis during fruit development and ripening of . Pairwise comparisons of two varieties at three developmental stages identified 365 differentially expressed lncRNAs in total. Functional annotation of target genes suggested that lncRNAs in may play roles in fruit color formation by regulating the expression of structural genes or regulatory factors. Construction of the regulatory network further revealed that the low expression of and may be the main cause of colorless fruit in .
为了探究果实发育和颜色变化的分子机制，采用比较转录组分析方法，在三个果实发育阶段（绿果期、转色期和成熟期）生成了两种典型野生品种的转录组图谱。我们在果实转录组中鉴定出了25,699个长链非编码RNA（lncRNA），它们来源于25,107个基因座，呈现出明显的阶段和基因型特异性表达模式。时间进程分析在这两个品种中均检测到大量与果实发育和成熟相关的差异表达蛋白质编码基因和lncRNA。在后期下调的靶基因在光合作用以及细胞壁组织或生物合成方面富集，这表明lncRNA可能作为负调控因子在果实发育和成熟过程中抑制光合作用以及细胞壁组织或生物合成。在三个发育阶段对两个品种进行两两比较，总共鉴定出365个差异表达的lncRNA。靶基因的功能注释表明，lncRNA可能通过调节结构基因或调控因子的表达在果实颜色形成中发挥作用。调控网络的构建进一步揭示，[具体基因1]和[具体基因2]的低表达可能是[品种名称]果实无色的主要原因。
