Department of Pharmacy, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.
College of Pharmacy, Youjiang Medical University for Nationalities, Baise, China.
J Cell Biochem. 2019 Aug;120(8):12677-12683. doi: 10.1002/jcb.28535. Epub 2019 Mar 10.
Hepatic function is of great concern in metabolic and immunological homeostasis. Traditionally, medical management to liver damage may benefit from phytomedicine, such as Chinese herbs. In southern China, Polygonum perfoliatum L can contribute to alleviating pathological symptoms of liver disease, such as hepatitis. However, bioactive compounds of hepatoprotection in this herb are still less to be investigated. In this study, clinical data of patients with drug-induced liver injury were collected on the basis of serological analyses. In addition, we extracted and identified total flavonoids from Polygonum perfoliatum L (TFPPL) before implementing biochemical experiments in vivo. In human data, the blood contents of liver function enzymes were visibly elevated, and the percentage of immune cells were abnormally changed. The data from the animal study showed that TFPPL-treated carbon tetrachloride-exposed mice resulted in reduced absolute liver mass and lowered blood levels of liver functional enzymes (alanine transaminase and aspartate transaminase). In enzyme-linked immunosorbent assay, the comparable data indicated that serological tumor necrosis factor α (TNF-α), interleukin 6, and heat shock protein 90 (Hsp90) contents were reduced in TFPPL-treated mice. In histopathological observations, TFPPL-treated mice exhibited reduced hepatocellular Hsp90, TNF-α, nuclear factor κ-light-chain-enhancer of activated B cells-p65 positive cells, and lowered Bax and caspase-3-labeled cells in the livers. Further, intrasplenic integrin β1, 5'-nucleotidase, and antigen KI-67 positive cells were increased after TFPPL treatments. Taken together, our present findings demonstrate that herb-extracted TFPPL exert potential hepatoprotective activities against chemical-induced liver damage in mice, possibly through the pharmacological mechanisms of inhibiting inflammatory stress and apoptosis, inactivating Hsp90 bioactivity in the liver, and improving splenic immunocompetence.
肝脏功能对于代谢和免疫稳态具有重要意义。传统上，针对肝损伤的医学治疗可能受益于植物药，如中药。在中国南方，贯叶连翘可缓解肝炎等肝病的病理症状。然而，这种草药中具有保肝作用的生物活性化合物仍有待进一步研究。本研究基于血清学分析收集了药物性肝损伤患者的临床数据，此外，我们从贯叶连翘中提取并鉴定了总黄酮（TFPPL），然后在体内进行生化实验。在人体数据中，肝功能酶的血液含量明显升高，免疫细胞的比例也发生了异常变化。动物研究数据表明，TFPPL 处理的四氯化碳暴露小鼠的绝对肝质量降低，肝功能酶（丙氨酸转氨酶和天冬氨酸转氨酶）的血液水平降低。在酶联免疫吸附试验中，可比数据表明 TFPPL 处理的小鼠血清肿瘤坏死因子 α（TNF-α）、白细胞介素 6 和热休克蛋白 90（Hsp90）含量降低。在组织病理学观察中，TFPPL 处理的小鼠肝脏中 Hsp90、TNF-α、核因子 κ-轻链增强子的 B 细胞 p65 阳性细胞、Bax 和 caspase-3 标记细胞减少，同时脾脏中整合素 β1、5'-核苷酸酶和抗原 KI-67 阳性细胞增加。总之，本研究结果表明，草药提取的 TFPPL 对小鼠化学诱导的肝损伤具有潜在的保肝活性，可能通过抑制炎症应激和细胞凋亡、使肝内 Hsp90 失去生物活性以及改善脾脏免疫功能的药理学机制发挥作用。
