分析重组巴西副球孢子菌 Gp70 在大肠杆菌中的一些免疫原性特性。
Analysis of some immunogenic properties of the recombinant Sporothrix schenckii Gp70 expressed in Escherichia coli.
机构信息
Departamento de Biología, División de Ciencias Naturales y Exactas, Campus Guanajuato, Universidad de Guanajuato, Noria Alta s/n, col. Noria Alta, C.P. 36050, Guanajuato, Gto., México.
Laboratory of Cellular Mycology & Proteomics, Universidade do Estado do Rio de Janeiro, Brazil.
出版信息
Future Microbiol. 2019 Mar;14:397-410. doi: 10.2217/fmb-2018-0295. Epub 2019 Mar 11.
AIM
Sporothrix schenckii is the causative agent of sporotrichosis. A 70-kDa glycoprotein, Gp70, is a candidate for the development of prophylactic alternatives to control the disease, and its gene (GP70) is predicted to encode for a protein of 43 kDa, contrasting with the molecular weight of the native protein.
MATERIALS & METHODS: The GP70 was expressed in bacteria, the recombinant protein purified, used in immunoassays and injected to Galleria mellonella.
RESULTS & CONCLUSION: The recombinant protein was detected by anti-Gp70 antibodies, confirming that the Gp70 backbone is a 43-kDa peptide. This protein showed enzyme activity of cyclase and was recognized by sera of patients with sporotrichosis. Although it was not useful for serodiagnosis of sporotrichosis, it conferred protection to animals against experimental sporotrichosis.
目的
申克孢子丝菌是孢子丝菌病的病原体。70kDa 糖蛋白（Gp70）是开发预防替代药物以控制该疾病的候选物，其基因（GP70）预测编码 43kDa 的蛋白质，与天然蛋白质的分子量形成对比。
材料与方法
在细菌中表达 GP70，纯化重组蛋白，用于免疫测定并注入金龟子幼虫。
结果与结论
重组蛋白被抗 Gp70 抗体检测到，证实 Gp70 主干是 43kDa 的肽。该蛋白显示环化酶的酶活性，并被孢子丝菌病患者的血清识别。尽管它对孢子丝菌病的血清学诊断没有帮助，但它为动物提供了针对实验性孢子丝菌病的保护。
Zotero 插件